[Method of antibody analysis based on visual protein array].

AIM

To establish a novel method for antibody analysis based on visual protein microarray.

METHODS

The antibodies spotted on the aldehyde-modified slides were incubated with corresponding antigens labeled by biotin, followed by silver enhancement detection method, and the chromogenic images were scanned by CCD camera.

RESULTS

The affinity and specificity of each antibody was assayed by this method and six antibodies were found to have higher specificity than the others. A detection limit of 0.4 microg/L was obtained for anti-globulinIIIC013, anti-albumin HPSIIB007, and anti-albumin HPSIIIB007.

CONCLUSION

This method is simple, fast and visual. In addition, it needs less sample amount than traditional immunoassay and has potential to achieve high throughput.