Li Caiwen, Shields Jeffrey D, Ratzlaff Robert E, Butler Mark J
Virginia Institute of Marine Science, The College of William and Mary, Gloucester Point, VA 23062, USA.
Virus Res. 2008 Mar;132(1-2):104-13. doi: 10.1016/j.virusres.2007.11.005. Epub 2008 Jan 4.
We examined the histopathological and hematological response of the Caribbean spiny lobster to experimentally induced infections with Panulirus argus Virus 1 (PaV1). The fixed phagocytes in the hepatopancreas were the primary sites of PaV1 infection in spiny lobsters. Fixed phagocytes were activated in early infections. However, as the disease progressed, the fixed phagocytes became infected and eventually lysed. Infected cells were subsequently observed in the hepatopancreas, gill, heart, hindgut, glial cells around the ventral nerves, and in the cuticular epidermis and foregut. In advanced infections, spongy connective tissues were heavily infected, as were glial cells around the optic nerves. The structure of the hepatopancreas was significantly altered as the disease progressed. The hemal sinuses among the hepatopancreatic tubules filled with massive amounts of cellular aggregates, including infected circulating hemocytes and spongy connective tissues. Atrophy of the hepatopancreatic tubules occurred in the late stage of viral infection. The virus caused significant decreases in total hemocyte counts and significantly altered several constituents in the hemolymph lysates of diseased lobsters, including: glucose, phosphorus, and triglycerides.
我们研究了加勒比刺龙虾对实验性感染的黄斑龙虾病毒1（PaV1）的组织病理学和血液学反应。肝胰腺中的固定吞噬细胞是刺龙虾中PaV1感染的主要部位。固定吞噬细胞在早期感染中被激活。然而，随着疾病的进展，固定吞噬细胞被感染并最终裂解。随后在肝胰腺、鳃、心脏、后肠、腹神经周围的神经胶质细胞以及表皮和前肠中观察到感染细胞。在晚期感染中，海绵状结缔组织以及视神经周围的神经胶质细胞受到严重感染。随着疾病的进展，肝胰腺的结构发生了显著改变。肝胰腺小管之间的血窦充满了大量细胞聚集体，包括受感染的循环血细胞和海绵状结缔组织。病毒感染后期肝胰腺小管出现萎缩。该病毒导致患病龙虾的总血细胞计数显著下降，并显著改变了血淋巴裂解物中的几种成分，包括葡萄糖､磷和甘油三酯。
