Kunwar A, Barik A, Mishra B, Rathinasamy K, Pandey R, Priyadarsini K I
Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India.
Biochim Biophys Acta. 2008 Apr;1780(4):673-9. doi: 10.1016/j.bbagen.2007.11.016. Epub 2007 Dec 15.
Using absorption and fluorescence spectroscopic methods, quantitative cellular uptake of curcumin, an antioxidant and anti-tumor agent from Curcuma longa, was calculated in two types of normal cells: spleen lymphocytes, and NIH3T3 and two tumor cell lines: EL4 and MCF7. Both the uptake and fluorescence intensity of curcumin were significantly higher in tumor cells compared to the normal cells. A linear dependency on the uptake was observed with treatment concentration of curcumin. Using laser confocal microscopy, intracellular localization of curcumin was monitored and the results indicated that curcumin is located both in the cell membrane and the nucleus. Sub-cellular fractionation of curcumin-loaded MCF7 cells supported the differential distribution of curcumin in membrane, cytoplasm and nuclear compartments of cell with maximum localization in the membrane. Cytotoxicity studies in different cell lines indicated that the toxicity of curcumin increased with increasing uptake.
利用吸收光谱法和荧光光谱法，计算了姜黄素（一种来自姜黄的抗氧化剂和抗肿瘤剂）在两种正常细胞（脾淋巴细胞和NIH3T3）以及两种肿瘤细胞系（EL4和MCF7）中的细胞摄取量。与正常细胞相比，肿瘤细胞中姜黄素的摄取量和荧光强度均显著更高。观察到姜黄素的摄取量与处理浓度呈线性相关。利用激光共聚焦显微镜监测了姜黄素的细胞内定位，结果表明姜黄素位于细胞膜和细胞核中。对负载姜黄素的MCF7细胞进行亚细胞分级分离，支持了姜黄素在细胞膜、细胞质和细胞核区室中的差异分布，其中在细胞膜中的定位最多。对不同细胞系的细胞毒性研究表明，姜黄素的毒性随摄取量增加而增加。
