Boudjema K, Cinqualbre J, Barguil Y, Pattou F, Kerr J A, Wolf P, Southard J H, Jaeck D
Service de Chirurgie générale et de Transplantations, Hôpital de Hautepierre, Strasbourg.
Chirurgie. 1991;117(5-6):372-9.
In this study we have investigated the effects of hepatocytes glycogen storage on the quality of livers for transplantation. Rats were fed or fasted for 24 h and hepatocytes isolated and cold stored in UW solution for 24 and 48 hours. Viability of the cells was analyzed by LDH release after 2 hours incubation in L15 with O2. Also, rabbits were fed, fasted (48 h) or glucose fed (48 h) and livers cold stored for 6, 24 and 48 h in UW solution. Functions of the livers were analyzed by isolated perfusion for 2 hours. Hepatocytes from fasted rats released significantly more LDH than hepatocytes from fed rats after 24 and 48 h cold storage. In rabbit livers, fasting depleted glycogen by 85% but had no effect on ATP or glutathione concentration. Livers from fasted rabbits produced similar amount of bile, released similar concentrations of lactate dehydrogenase and aspartate transaminase into the perfusate, maintained similar concentrations of glutathione after 24 hours preservation when compared to fed animals. After 48 h preservation livers from fasted animals were less viable than livers from fed animals and the decrease of liver functions in livers from fasted animals preserved for 48 hours was prevented by feeding glucose. This study shows that liver glycogen storage in hepatocyte is an important metabolite for successful liver preservation. Glycogen may be a source for ATP and antioxydant synthesis during the early period of reperfusion.
在本研究中，我们调查了肝细胞糖原储存对肝脏移植质量的影响。将大鼠喂食或禁食24小时，分离肝细胞并在UW溶液中冷藏24小时和48小时。在含O₂的L15中孵育2小时后，通过乳酸脱氢酶（LDH）释放分析细胞活力。此外，将兔子喂食、禁食（48小时）或喂食葡萄糖（48小时），并将肝脏在UW溶液中冷藏6小时、24小时和48小时。通过离体灌注2小时分析肝脏功能。冷藏24小时和48小时后，禁食大鼠的肝细胞释放的LDH明显多于喂食大鼠的肝细胞。在兔肝脏中，禁食使糖原减少85%，但对ATP或谷胱甘肽浓度没有影响。与喂食动物相比，禁食兔子的肝脏在保存24小时后产生的胆汁量相似，向灌注液中释放的乳酸脱氢酶和天冬氨酸转氨酶浓度相似，谷胱甘肽浓度维持相似。保存48小时后，禁食动物的肝脏活力低于喂食动物的肝脏，喂食葡萄糖可防止保存48小时的禁食动物肝脏的肝功能下降。本研究表明，肝细胞中的肝脏糖原储存是肝脏成功保存的重要代谢物。糖原可能是再灌注早期ATP和抗氧化剂合成的来源。
