Watanabe-Kamiyama Mari, Kamiyama Shin, Horiuchi Kimiko, Ohinata Kousaku, Shirakawa Hitoshi, Furukawa Yuji, Komai Michio
Laboratory of Nutrition, Department of Science of Food Function and Health, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
Br J Nutr. 2008 Apr;99(4):756-63. doi: 10.1017/S0007114507841122. Epub 2008 Jan 8.
Biotin is a member of the vitamin B-complex family. Biotin deficiency has been associated with hyperglycaemia and insulin resistance in animals and humans. In the present study, we investigated the pharmacological effects of biotin on hypertension in the stroke-prone spontaneously hypertensive rat (SHRSP) strain. We observed that long-term administration of biotin decreased systolic blood pressure in the SHRSP strain; also, a single dose of biotin immediately decreased systolic blood pressure in this strain. Pretreatment with the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazole [4,3-alpha]quinoxalin-1-one abolished the hypotensive action of biotin in the SHRSP strain, while pretreatment with the NO synthase inhibitor NG-nitro-l-arginine methyl ester had no effect on the action of biotin. Biotin reduced coronary arterial thickening and the incidence of stroke in the SHRSP strain. These results suggest that the pharmacological dose of biotin decreased the blood pressure of the SHRSP via an NO-independent direct activation of soluble guanylate cyclase. Our findings reveal the beneficial effects of biotin on hypertension and the incidence of stroke.
生物素是复合维生素B家族的一员。在动物和人类中，生物素缺乏与高血糖症和胰岛素抵抗有关。在本研究中，我们调查了生物素对易中风自发性高血压大鼠（SHRSP）品系高血压的药理作用。我们观察到，长期给予生物素可降低SHRSP品系的收缩压；此外，单次给予生物素可立即降低该品系的收缩压。用鸟苷酸环化酶抑制剂1H-[1,2,4]恶二唑[4,3-α]喹喔啉-1-酮预处理可消除生物素对SHRSP品系的降压作用，而用一氧化氮合酶抑制剂NG-硝基-L-精氨酸甲酯预处理对生物素的作用没有影响。生物素可减轻SHRSP品系的冠状动脉增厚和中风发生率。这些结果表明，药理剂量的生物素通过不依赖一氧化氮的可溶性鸟苷酸环化酶直接激活来降低SHRSP的血压。我们的研究结果揭示了生物素对高血压和中风发生率的有益作用。
