Patraki E, Cardillo M R
Department of Experimental Medicine and Pathology, Section of Pathologic Anatomy-Uropathology Unit, University La Sapienza, Rome, Italy.
Int J Immunopathol Pharmacol. 2007 Oct-Dec;20(4):697-705. doi: 10.1177/039463200702000405.
The role of matrilysin 1 or matrix metalloproteinase-7 (MMP-7) in cancer is extremely complex and poorly understood. In this study we investigated differential expression of MMP-7 in the epithelium and stroma of 95 paraffin-embedded renal tumor samples by immunohistochemistry and compared tumoral with normal peritumoral renal tissue. We also determined a possible correlation of the immunohistochemical findings with histological subtype, tumor grade and stage of RCC. In all areas examined MMP-7 protein expression was significantly higher in epithelium than in stroma (P less than 0.01). MMP-7 was more less expressed in peritumoral normal areas than in benign epithelial neoplasias (renal papillary and oncocytomas) and RCC carcinomas, reaching the highest immunopositive reaction in chromophobe RCC subtypes, followed by conventional clear-cell and chromophilic-papillary RCC histological subtypes and the lowest levels in more aggressive RCC histotypes (spindle-cell and collecting-duct RCCs). MMP-7 reached their highest levels in high-grade and high-stage RCCs. Our observation suggests an important role of MMP-7 in the development and progression of renal cancer. The differential expression of MMP-7 in the various histological RCC subtypes may reflect the malignant phenotype and more aggressive behavior of RCCs.
基质溶素1或基质金属蛋白酶-7（MMP-7）在癌症中的作用极其复杂，目前了解甚少。在本研究中，我们通过免疫组织化学方法研究了95例石蜡包埋的肾肿瘤样本上皮和基质中MMP-7的差异表达，并将肿瘤组织与肿瘤周围正常肾组织进行了比较。我们还确定了免疫组织化学结果与肾细胞癌（RCC）的组织学亚型、肿瘤分级和分期之间可能存在的相关性。在所有检查区域，上皮中MMP-7蛋白表达显著高于基质（P小于0.01）。肿瘤周围正常区域的MMP-7表达低于良性上皮性肿瘤（肾乳头状瘤和嗜酸细胞瘤）和RCC癌，在嫌色性RCC亚型中免疫阳性反应最高，其次是传统透明细胞和嗜色性乳头状RCC组织学亚型，在侵袭性更强的RCC组织类型（梭形细胞和集合管RCC）中水平最低。MMP-7在高级别和高分期RCC中表达水平最高。我们的观察结果表明MMP-7在肾癌的发生和发展中起重要作用。MMP-7在各种组织学RCC亚型中的差异表达可能反映了RCC的恶性表型和更强的侵袭性。
