全反式维甲酸对人角质形成细胞和口腔癌表皮样细胞培养物生长的不同影响。GRIM-19与呼吸链复合体I的参与。

Differential effects of all-trans retinoic acid on the growth of human keratinocytes and mouth carcinoma epidermoid cultures. Involvement of GRIM-19 and complex I of the respiratory chain.

作者信息

Papa F, Delia M, Trentadue R, Panelli D, Bellomo F, Serpico R, Petruzzi M, De Benedittis M, Scacco S

机构信息

Department of Odontostomatology and Surgery, University of Bari, Italy.

出版信息

Int J Immunopathol Pharmacol. 2007 Oct-Dec;20(4):719-29. doi: 10.1177/039463200702000407.

DOI:10.1177/039463200702000407

PMID:18179744

Abstract

Squamous cell carcinoma (SSC) is the most frequent malignant tumor of the oral cavity. A study on the effect of all-trans retinoic acid (RA) on cell growth, expression of GRIM-19 and content and activity of complex I of the mitochondrial respiratory chain in normal human keratinocytes (NHEK) and mouth carcinoma cells with low (HN) and high (KB) transformation grade was carried out. In NHEK cells, RA treatment resulted in growth suppression, significant overexpression of GRIM-19 protein, enhanced content of complex I but depressed activity of NADH-UQ oxidoreductase activity of the complex. In HN cells, RA treatment depressed cell growth, inhibited the enzymatic activity of complex I but had no significant effect on the levels of GRIM-19 and complex I. In KB cells RA had no effect on cell growth, GRIM-19 expression, content and activity of complex I.

摘要

鳞状细胞癌（SSC）是口腔中最常见的恶性肿瘤。开展了一项关于全反式维甲酸（RA）对正常人角质形成细胞（NHEK）以及低转化等级（HN）和高转化等级（KB）口腔癌细胞的细胞生长、GRIM-19表达以及线粒体呼吸链复合体I的含量和活性影响的研究。在NHEK细胞中，RA处理导致生长抑制、GRIM-19蛋白显著过表达、复合体I含量增加，但复合体的NADH-UQ氧化还原酶活性降低。在HN细胞中，RA处理抑制细胞生长，抑制复合体I的酶活性，但对GRIM-19和复合体I的水平无显著影响。在KB细胞中，RA对细胞生长、GRIM-19表达、复合体I的含量和活性均无影响。