Ishizawa T, Hayashi M, Awazu S
Pharmaceutical Research Laboratories, Meiji Seika Kaisha, Ltd., Yokohama, Japan.
J Pharmacobiodyn. 1991 Oct;14(10):583-9. doi: 10.1248/bpb1978.14.583.
The permeability of fosfomycin (FOM), which is a low molecular weight and water-soluble antibiotic, across rat and rabbit jejunal membrane was investigated on a paracellular route and a transcellular route separated by voltage-clamp method. It was determined that FOM permeates through these two routes in both animals. Under a physiologically normal condition, the transmucosal electrical potential difference (about 2 mV), the contribution of the transcellular route to FOM membrane permeability was greater than that of the paracellular route. The transcellular permeability was significantly reduced in the presence of inorganic phosphate. The inhibitory effects of the inorganic phosphate coincided with our reports on FOM uptake by rat jejunal brush-border membrane vesicles in vitro and on FOM absorption by rat jejunal single-pass perfusion in situ.
磷霉素（FOM）是一种低分子量的水溶性抗生素，采用电压钳法分别研究了其在大鼠和家兔空肠黏膜的细胞旁路途径和跨细胞途径的通透性。结果表明，在这两种动物中FOM均可通过这两条途径渗透。在生理正常条件下，跨黏膜电位差（约2 mV）时，FOM的跨细胞途径对膜通透性的贡献大于细胞旁路途径。无机磷酸盐存在时，跨细胞通透性显著降低。无机磷酸盐的抑制作用与我们之前关于大鼠空肠刷状缘膜囊泡对FOM的摄取以及大鼠空肠原位单通道灌注对FOM吸收的报道一致。
