Khurana Harpreet Kaur, Cho Il Kyu, Shim Jae Yong, Li Qing X, Jun Soojin
Department of Human Nutrition, Food and Animal Science and Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii 96822, USA.
J Agric Food Chem. 2008 Feb 13;56(3):778-83. doi: 10.1021/jf0724116. Epub 2008 Jan 9.
Aspartame is a low-calorie sweetener commonly used in soft drinks; however, the maximum usage dose is limited by the U.S. Food and Drug Administration. Fourier transform infrared (FTIR) spectroscopy with attenuated total reflectance sampling accessory and partial least-squares regression (PLS) was used for rapid determination of aspartame in soft drinks. On the basis of spectral characterization, the highest R2 value, and lowest PRESS value, the spectral region between 1600 and 1900 cm(-1) was selected for quantitative estimation of aspartame. The potential of FTIR spectroscopy for aspartame quantification was examined and validated by the conventional HPLC method. Using the FTIR method, aspartame contents in four selected carbonated diet soft drinks were found to average from 0.43 to 0.50 mg/mL with prediction errors ranging from 2.4 to 5.7% when compared with HPLC measurements. The developed method also showed a high degree of accuracy because real samples were used for calibration, thus minimizing potential interference errors. The FTIR method developed can be suitably used for routine quality control analysis of aspartame in the beverage-manufacturing sector.
阿斯巴甜是一种常用于软饮料的低热量甜味剂；然而，其最大使用剂量受到美国食品药品监督管理局的限制。采用带有衰减全反射采样附件的傅里叶变换红外（FTIR）光谱法和偏最小二乘回归（PLS）法对软饮料中的阿斯巴甜进行快速测定。基于光谱特征、最高的R2值和最低的预测残差平方和（PRESS）值，选择1600至1900 cm(-1)的光谱区域用于阿斯巴甜的定量估算。通过传统的高效液相色谱（HPLC）方法对FTIR光谱法定量阿斯巴甜的潜力进行了考察和验证。使用FTIR方法，发现四种选定的碳酸低热量软饮料中的阿斯巴甜含量平均为0.43至0.50 mg/mL，与HPLC测量结果相比，预测误差范围为2.4%至5.7%。所开发的方法还显示出高度的准确性，因为使用实际样品进行校准，从而最大限度地减少了潜在的干扰误差。所开发的FTIR方法可适用于饮料制造行业中阿斯巴甜的常规质量控制分析。
