Hayashi Yoshihito, Oshige Ikuya, Katsumoto Yoichi, Omori Shinji, Yasuda Akio, Asami Koji
Life Science Laboratory, Materials Laboratories, Sony Corporation, Sony Bioinformatics Center, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan.
Phys Med Biol. 2008 Jan 7;53(1):295-304. doi: 10.1088/0031-9155/53/1/021. Epub 2007 Dec 19.
Rabbit blood was preserved at 277 K in Alsever's solution for 37 days, and its dielectric permittivity was monitored in a frequency range from 0.05 to 110 MHz throughout the period. The relaxation time and Cole-Cole parameter of the interfacial polarization process for erythrocytes remained nearly constant during the first 20 days and then started to increase and decrease, respectively. On the other hand, the relaxation strength and the cell volume fraction continued to decrease for 37 days, but the decrease rates of both changed discontinuously on about the 20th day. Microscope observation showed that approximately 90% of the erythrocytes were spinous echinocytes at the beginning of preservation and started to be transformed into microspherocytes around the 20th day. Therefore, dielectric spectroscopy is a sensitive tool to monitor the deterioration of preserved blood accompanied by morphological transition of erythrocytes through the temporal variation of their dielectric properties.
兔血在277 K下于阿氏液中保存37天，在此期间，其介电常数在0.05至110 MHz的频率范围内进行监测。红细胞界面极化过程的弛豫时间和科尔 - 科尔参数在前20天内几乎保持恒定，然后分别开始增加和减少。另一方面，弛豫强度和细胞体积分数在37天内持续下降，但两者的下降速率在大约第20天出现不连续变化。显微镜观察表明，保存开始时约90%的红细胞为棘状棘红细胞，在第20天左右开始转变为微球形红细胞。因此，介电谱是一种通过血液介电特性随时间的变化来监测保存血液变质以及红细胞形态转变的灵敏工具。
