市售番茄提取物在番茄过敏诊断中的效用：与点刺法的比较。

Usefulness of manufactured tomato extracts in the diagnosis of tomato sensitization: Comparison with the prick-prick method.

作者信息

Ferrer Angel, Huertas Angel J, Larramendi Carlos H, García-Abujeta Jose L, Bartra Joan, Lavín Jose R, Andreu Carmen, Pagán Juan A, López-Matas María A, Fernández-Caldas Enrique, Carnés Jerónimo

机构信息

Research & Development Department, Laboratorios LETI, S,L,, Tres Cantos, Madrid, Spain.

出版信息

Clin Mol Allergy. 2008 Jan 9;6:1. doi: 10.1186/1476-7961-6-1.

DOI:10.1186/1476-7961-6-1

PMID:18184431

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2263074/

Abstract

BACKGROUND

Commercial available skin prick test with fruits can be negative in sensitized or allergic patients due to a reduction in biological activity during the manufacturing process. Prick-prick tests with fresh foods are often preferred, but they are a non-standardized procedure. The usefulness of freeze-dried extracts of Canary Islands tomatoes, comparing the wheal sizes induced by prick test with the prick-prick method in the diagnosis of tomato sensitization has been analyzed.The objective of the study was to assess the potential diagnostic of freeze-dried extracts of Canary Islands tomatoes, comparing the wheal sizes induced by prick test with the prick-prick method.

METHODS

Two groups of patients were analyzed: Group I: 26 individuals reporting clinical symptoms induced by tomato contact or ingestion. Group II: 71 control individuals with no symptoms induced by tomato: 12 of them were previously skin prick test positive to a tomato extract, 39 were atopic and 20 were non-atopic. All individuals underwent prick-prick with fresh ripe peel Canary tomatoes and skin prick tested with freeze-dried peel and pulp extracts obtained from peel and pulp of Canary tomatoes at 10 mg/ml. Wheal sizes and prick test positivity (>/= 7 mm2) were compared between groups.

RESULTS

In group I, 21 (81%) out of 26 patients were prick-prick positive. Twenty patients (77%) had positive skin prick test to peel extracts and 12 (46%) to pulp extracts. Prick-prick induced a mean wheal size of 43.81 +/- 40.19 mm2 compared with 44.25 +/- 36.68 mm2 induced by the peel extract (Not significant), and 17.79 +/- 9.39 mm2 induced by the pulp extract (p < 0.01).In group II, 13 (18%) out of 71 control patients were prick-prick positive. Twelve patients (all of them previously positive to peel extract) had positive skin prick test to peel and 3 to pulp. Prick-prick induced a mean wheal size of 28.88 +/- 13.12 mm2 compared with 33.17 +/- 17.55 mm2 induced by peel extract (Not significant), and 13.33 +/- 4.80 mm2 induced by pulp extract (p < 0.05 with peel extract and prick-prick).

CONCLUSION

Canary peel tomato extract seems to be as efficient as prick-prick tests with ripe tomatoes to diagnose patients sensitized to tomato. The wheal sizes induced by prick-prick and peel extracts were very similar and showed a high correlation coefficient.

摘要

背景

市售的水果皮肤点刺试验在致敏或过敏患者中可能呈阴性，原因是制造过程中生物活性降低。新鲜食物的点刺-点刺试验通常更受青睐，但这是一种非标准化程序。分析了加那利群岛番茄冻干提取物的效用，比较了点刺试验与点刺-点刺法诱导的风团大小在番茄致敏诊断中的作用。本研究的目的是评估加那利群岛番茄冻干提取物的潜在诊断价值，比较点刺试验与点刺-点刺法诱导的风团大小。

方法

分析两组患者：第一组：26名报告有番茄接触或摄入引起临床症状的个体。第二组：71名无番茄引起症状的对照个体：其中12人先前对番茄提取物皮肤点刺试验呈阳性，39人是特应性个体，20人是非特应性个体。所有个体均用新鲜成熟的加那利番茄果皮进行点刺-点刺试验，并用从加那利番茄果皮和果肉中获得的10mg/ml冻干果皮和果肉提取物进行皮肤点刺试验。比较两组之间的风团大小和点刺试验阳性率（≥7mm²）。

结果

在第一组中，26名患者中有21名（81%）点刺-点刺试验呈阳性。20名患者（77%）对果皮提取物皮肤点刺试验呈阳性，12名患者（46%）对果肉提取物呈阳性。点刺-点刺法诱导的平均风团大小为43.81±40.19mm²，而果皮提取物诱导的为44.25±36.68mm²（无显著性差异），果肉提取物诱导的为17.79±9.39mm²（p<0.01）。在第二组中，71名对照患者中有13名（18%）点刺-点刺试验呈阳性。12名患者（均先前对果皮提取物呈阳性）对果皮皮肤点刺试验呈阳性，3名对果肉呈阳性。点刺-点刺法诱导的平均风团大小为28.88±13.12mm²，而果皮提取物诱导的为33.17±17.55mm²（无显著性差异），果肉提取物诱导的为13.33±4.80mm²（与果皮提取物和点刺-点刺法相比，p<0.05）。