Kemény E, Nádasdy T, Szenohradszky P, Csajbók E, Ormos J
Department of Pathology, Szent-Györgyi Albert Medical University, Szeged, Hungary.
Acta Morphol Hung. 1991;39(3):177-86.
Composition of the extracellular matrix (ECM) was studied in transplant vasculopathy occurring in rejected renal allografts using the immunoperoxidase technique with antisera against laminin, and collagen types I, III and IV. In acute transplant vasculopathy the loose ECM network of the intima showed intense immunostaining for laminin and type IV collagen. Type III collagen was detected in the advanced acute cellular intimal proliferations, while early acute lesions did not show immunreactions. Type I collagen was not seen in significant amount. In contrast to these findings in chronic transplant vasculopathy associated with intimal fibrosis the ECM was largely composed of interstitial collagen types III and I, while staining for the basement membrane type ECM components were markedly reduced. Degradation of the matrix components with variable composition was noted in foci of mononuclear infiltrates occurring inside the fibrotic intima. These results indicate that the ECM shows a compositional change in transplant vasculopathy which is associated with the age of the lesion.
采用抗层粘连蛋白、Ⅰ型、Ⅲ型和Ⅳ型胶原抗血清的免疫过氧化物酶技术，对移植肾排斥反应中发生的移植血管病的细胞外基质（ECM）组成进行了研究。在急性移植血管病中，内膜疏松的ECM网络对层粘连蛋白和Ⅳ型胶原呈强烈免疫染色。Ⅲ型胶原在晚期急性细胞内膜增生中被检测到，而早期急性病变未显示免疫反应。未发现大量的Ⅰ型胶原。与伴有内膜纤维化的慢性移植血管病的这些发现相反，ECM主要由Ⅲ型和Ⅰ型间质胶原组成，而基底膜型ECM成分的染色明显减少。在纤维化内膜内出现的单核浸润灶中，注意到组成成分各异的基质成分发生降解。这些结果表明，ECM在移植血管病中表现出与病变年龄相关的组成变化。
