在斯坦尼斯拉斯家族研究中，脂蛋白脂肪酶丝氨酸447位点突变与血清类胡萝卜素浓度改变有关。

The lipoprotein lipase serine 447 stop polymorphism is associated with altered serum carotenoid concentrations in the Stanislas Family Study.

作者信息

Herbeth Bernard, Gueguen Sonia, Leroy Pierre, Siest Gérard, Visvikis-Siest Sophie

机构信息

INSERM U525, Université Henri Poincaré, Faculté de Pharmacie, 30 rue Lionnois, F54000 Nancy, France.

出版信息

J Am Coll Nutr. 2007 Dec;26(6):655-62. doi: 10.1080/07315724.2007.10719644.

DOI:10.1080/07315724.2007.10719644

PMID:18187430

Abstract

BACKGROUND

Carotenoids are mainly carried by lipoproteins in blood, however little is known about the influence of polymorphisms of apolipoproteins (apo), cholesterol ester transfer protein (CETP) and lipoprotein lipase (LPL) involved in serum lipid metabolism.

OBJECTIVE

We aimed to analyze whether serum concentrations of 5 carotenoids (lutein-zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, beta-carotene) are associated with common polymorphisms of Apo E, Apo B, Apo CIII, CETP, and LPL.

METHODS

Serum concentrations of lutein-zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, and beta-carotene were measured and polymorphisms of Apo E (cys112arg and arg158cys), Apo B (thr71ile), Apo CIII [C(-482)T, Apo CIII T(-455)C, Apo CIII C1100T, Apo CIII C3175G, Apo CIII T3206G], CETP (ile405val), and LPL (S447X) were determined in a sample of 447 children and adults drawn from the Stanislas Study.

RESULTS

After adjustment for age, sex, smoking, physical activity, oral contraceptive use, BMI, serum cholesterol and triglyceride concentrations, and fruit and vegetable intakes, carriers vs. non carriers of the lipoprotein lipase X447 allele had significant lower concentrations of lutein-zeaxanthin, beta-cryptoxanthin, alpha-carotene and beta-carotene; differences vs. S447S genotype being the largest for X447X: -18.8%, -50.5%, -54.8% and -47.1%, for the four carotenoid fractions, respectively. No significant association was noticed for lycopene concentration. None of the other tested polymorphisms was significantly related to the serum carotenoid concentrations.

CONCLUSIONS

Our investigation for the first time demonstrates that LPL S447X polymorphism could alter serum concentrations of carotenoids in healthy individuals, independently of serum cholesterol and triglyceride concentration. These data indicate that genetic factors could be involved in the variability of carotenoid bioavailability and bioconversion.

摘要

背景

类胡萝卜素在血液中主要由脂蛋白携带，然而，关于参与血清脂质代谢的载脂蛋白（apo）、胆固醇酯转运蛋白（CETP）和脂蛋白脂肪酶（LPL）多态性的影响知之甚少。

目的

我们旨在分析5种类胡萝卜素（叶黄素-玉米黄质、β-隐黄质、番茄红素、α-胡萝卜素、β-胡萝卜素）的血清浓度是否与载脂蛋白E、载脂蛋白B、载脂蛋白CIII、CETP和LPL的常见多态性相关。

方法

在从斯坦尼斯拉斯研究中抽取的447名儿童和成人样本中，测量了叶黄素-玉米黄质、β-隐黄质、番茄红素、α-胡萝卜素和β-胡萝卜素的血清浓度，并确定了载脂蛋白E（cys112arg和arg158cys）、载脂蛋白B（thr71ile）、载脂蛋白CIII [C(-482)T、载脂蛋白CIII T(-455)C、载脂蛋白CIII C1100T、载脂蛋白CIII C3175G、载脂蛋白CIII T3206G]、CETP（ile405val）和LPL（S447X）的多态性。

结果

在调整年龄、性别、吸烟、身体活动、口服避孕药使用、BMI、血清胆固醇和甘油三酯浓度以及水果和蔬菜摄入量后，脂蛋白脂肪酶X447等位基因的携带者与非携带者相比，叶黄素-玉米黄质、β-隐黄质、α-胡萝卜素和β-胡萝卜素的浓度显著降低；与S447S基因型相比，X447X基因型在四种类胡萝卜素组分中的差异最大，分别为-18.8%、-50.5%、-54.8%和-47.1%。未发现番茄红素浓度有显著关联。其他测试的多态性均与血清类胡萝卜素浓度无显著相关性。