Shpakov A A, Dudchenko A M, Dudchenko V K
Tsitologiia. 1976 Mar;18(3):312-8.
Activities of succinate dehydrogenase, succinate- and NAD-H-cytochrome c--reductases, and cytochrome c--oxidase was compared in 1 g tissue homogenate and homogenate fractions made from 1 g brain tissue using various solutions. Fractionation resulted in the increased activities of NADH- and succinate cytochrome reductases, and in the loss of succinate dehydrogenase activity, cytochrome oxidase was less influenced. These phenomena are regarded as signs of the interrelation between mitochondria and other constituents of brain cell within homogenates. Maximal quantity of mitochondria isolated from homogenates is no more than 20% of all the mitochondrial homogenates (according enzyme data). The electronogram of the brain mitochondrial preparation isolated in the Krebs--Ringer solution without glucose pointed out to a high homogeneity of mitochondria in the residue.
使用不同溶液，比较了1g脑组织制成的组织匀浆及其匀浆组分中琥珀酸脱氢酶、琥珀酸和NAD - H - 细胞色素c还原酶以及细胞色素c氧化酶的活性。分级分离导致NADH和琥珀酸细胞色素还原酶活性增加，琥珀酸脱氢酶活性丧失，细胞色素氧化酶受影响较小。这些现象被视为匀浆中线粒体与脑细胞其他成分之间相互关系的标志。从匀浆中分离出的线粒体最大量不超过所有线粒体匀浆的20%（根据酶数据）。在不含葡萄糖的Krebs - Ringer溶液中分离的脑线粒体制剂的电子显微镜照片表明，残渣中线粒体具有高度同质性。
