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[不同波长紫外线对扎伊德尔腹水肝癌细胞中DNA-品红浓度的影响]

[The effect of ultraviolet radiation of different wave lengths on the concentration of DNA--fuchsin in the cells of Zajdel's ascitic hepatoma].

作者信息

Krylenkov V A, Arkhipov M V, Samoĭlova K A

出版信息

Tsitologiia. 1976;18(5):639-43.

PMID:181882
Abstract

The cytophotometrical analysis of the relationships between the content of DNA--fuchsin and the duration of acid hydrolysis of the intranuclear DNA (the Feulgen reaction) in the Zaidela hepatoma cells allows to see the degree of the DNA--protein binding. The UV irradiation of different wave-lengths (254, 325 and 365 nm, resp.) irrespective of the degree of absorption by nucleic acids, causes changes in chromatin condensation, thus suggesting the labilization of DNA--protein bounds.

摘要

对扎伊德拉肝癌细胞中DNA-品红含量与核内DNA酸水解持续时间(福尔根反应)之间关系的细胞光度分析,有助于了解DNA-蛋白质结合的程度。不同波长(分别为254、325和365纳米)的紫外线照射,无论核酸的吸收程度如何,都会引起染色质凝聚的变化,从而表明DNA-蛋白质结合变得不稳定。

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