使用二茂铁共轭ATP对激酶催化的磷酸化进行电化学检测。
Electrochemical detection of kinase-catalyzed phosphorylation using ferrocene-conjugated ATP.
作者信息
Song Haifeng, Kerman Kagan, Kraatz Heinz-Bernhard
机构信息
Department of Chemistry, University of Western Ontario, London, Ontario, Canada.
出版信息
Chem Commun (Camb). 2008 Jan 28(4):502-4. doi: 10.1039/b714383d. Epub 2007 Nov 12.
DOI:10.1039/b714383d
PMID:18188482
Abstract
Adenosine-5'-[gamma-ferrocene] triphosphate is exploited as a co-substrate for the phosphorylation of the surface-immobilized peptide C-SIYRRGSRRWRKL by protein kinase C, in which the gamma-ferrocene phosphate is transferred to the peptide and then detected by cyclic voltammetry.
摘要
5'-[γ-二茂铁基]三磷酸腺苷被用作蛋白激酶C对表面固定化肽C-SIYRRGSRRWRKL进行磷酸化的共底物，其中γ-二茂铁基磷酸转移到该肽上，然后通过循环伏安法进行检测。
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