Jelski W, Zalewski B, Chrostek L, Szmitkowski M
Department of Biochemical Diagnostics, Medical Academy, Waszyngtona 15A, PL-15-276, Bialystok, Poland.
Clin Exp Med. 2007 Dec;7(4):154-7. doi: 10.1007/s10238-007-0140-0. Epub 2008 Jan 11.
Various isoenzymes of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) exist in human colorectal mucosa. In our last experiments we have shown that ADH and ALDH are present also in colorectal cancer cells. Moreover the activities of total ADH and class I isoenzymes were significantly higher in cancer tissue than healthy mucosa. This may suggest that these changes may be reflected by enzyme activity in the serum. Therefore, we have measured the activity of total ADH, and classes I-IV of this enzyme and ALDH in the sera of patients suffering from this cancer. Total ADH activity was measured by a photometric method with p-nitrosodimethylaniline (NDMA) as a substrate and ALDH activity by the fluorometric method with 6-methoxy-2-naphtaldehyde as a substrate. For the measurement of the activity of class I and II isoenzymes we employed fluorometric methods, with class-specific fluorogenic substrates. The activity of class III ADH was measured by the photometric method with formaldehyde and class IV with m-nitrobenzaldehyde as a substrate. Serum samples were taken for routine biochemical investigations from 52 patients with colorectal carcinoma before treatment. A statistically significant increase of class I ADH isoenzymes was found. Therefore the total ADH activity was also significantly increased. The total ALDH and the activity of other tested ADH isoenzymes were unchanged. We also observed the increasing tendency of ADH I activity in accordance with the advance of disease. The activity of class I ADH isoenzymes was elevated in the serum of patients with colorectal cancer. This activity was derived from colorectal cancer cells and probably from severely damaged liver by metastatic disease.
酒精脱氢酶（ADH）和醛脱氢酶（ALDH）的多种同工酶存在于人大肠黏膜中。在我们之前的实验中，我们已经表明ADH和ALDH也存在于大肠癌细胞中。此外，癌组织中总ADH和I类同工酶的活性明显高于健康黏膜。这可能表明这些变化可能反映在血清中的酶活性上。因此，我们测量了患有这种癌症的患者血清中总ADH及其I-IV类酶和ALDH的活性。总ADH活性通过以对亚硝基二甲基苯胺（NDMA）为底物的光度法测量，ALDH活性通过以6-甲氧基-2-萘醛为底物的荧光法测量。对于I类和II类同工酶活性的测量，我们采用荧光法，使用类特异性荧光底物。III类ADH的活性通过以甲醛为底物的光度法测量，IV类通过以间硝基苯甲醛为底物的光度法测量。在治疗前从52例结直肠癌患者中采集血清样本进行常规生化检查。发现I类ADH同工酶有统计学意义的增加。因此总ADH活性也显著增加。总ALDH和其他测试的ADH同工酶的活性没有变化。我们还观察到随着疾病进展ADH I活性有增加的趋势。结直肠癌患者血清中I类ADH同工酶的活性升高。这种活性来自结直肠癌细胞，可能也来自转移性疾病严重受损的肝脏。
