Oliveira P G, Brenol C V, Edelweiss M I, Meurer L, Brenol J C T, Xavier R M
Medical Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
Clin Exp Med. 2007 Dec;7(4):184-7. doi: 10.1007/s10238-007-0148-6. Epub 2008 Jan 11.
Complete Freund's adjuvant (CFA)-induced arthritis in rats, which presents similar features to rheumatoid arthritis, is a model widely used in aetiopathogenetic and investigational drug studies. In this model, arthritis is induced by intradermal injection of Mycobacterium tuberculosis suspended in mineral oil in the hind footpad. Although the histopathology findings in the joint are well described, the marked subcutaneous features of panniculitis that concomitantly occur in this model have received no attention. The objective of this paper is to describe the subcutaneous histopathological features in 8 Wistar rats after intraplantar injection of CFA. We studied the subcutaneous histopathological features in 8 Wistar rats after intraplantar injection of CFA in the left hind paw. The levels of subcutaneous inflammation of the animals in this study were evaluated for the histological characteristics present in the tissue and scored with 4 parameters (acute inflammation, chronic inflammation with fibrosis, subcutaneous and profound soft tissue necrosis, and the presence of giant cells, neutrophils, macrophages and lymphocytes) on days 4, 7, 11 and 15 after induction. All animals developed intense subcutaneous inflammation characteristic of panniculitis, with predominance of acute changes in the initial period, with progression to a self-perpetuating chronic fibrotic process on day 15. These observations precede the joint changes. Besides being an interesting model for better studying diseases with panniculitis, our observations bring up issues concerning the possible relations between subcutaneous and joint inflammatory changes.
完全弗氏佐剂（CFA）诱导的大鼠关节炎与类风湿关节炎具有相似特征，是病因发病机制和药物研究中广泛使用的模型。在该模型中，通过在后足垫皮内注射悬浮于矿物油中的结核分枝杆菌诱导关节炎。尽管关节的组织病理学发现已有详尽描述，但该模型中同时出现的明显皮下脂膜炎特征却未受到关注。本文的目的是描述8只Wistar大鼠足底注射CFA后的皮下组织病理学特征。我们研究了8只Wistar大鼠左后爪足底注射CFA后的皮下组织病理学特征。在诱导后第4、7、11和15天，根据组织中存在的组织学特征评估本研究中动物的皮下炎症水平，并用4个参数（急性炎症、伴有纤维化的慢性炎症、皮下和深部软组织坏死以及巨细胞、中性粒细胞、巨噬细胞和淋巴细胞的存在情况）进行评分。所有动物均出现了脂膜炎特有的强烈皮下炎症，初期以急性变化为主，到第15天发展为持续的慢性纤维化过程。这些观察结果先于关节变化出现。除了是更好地研究脂膜炎相关疾病的有趣模型外，我们的观察结果还提出了关于皮下和关节炎症变化之间可能关系的问题。
