Intrinsic catalytic activity of procarboxypeptidase A. A kinetic study using fluorine analogues.

Bovine procarboxypeptidase A displays substantial catalytic activity toward halogenated acyl-amino acids, the most active of which is trifluoroacetyl-L-phenylalanine (TFAc-L-Phe). Though this activity is not as great as for the native enzyme, it is quite substantial and far beyond the range of adventitious activation. Both DL-benzylcuccinate and beta-phenylpropionate inhibit zymogen hydrolysis of TFAc-L-Phe, the former with a K1 of 4.1 micrometer and the latter, 900 micrometer (a value much higher than the corresponding enzyme). Apo procarboxypeptidase A will also hydrolyze TFAc-L-Phe, presumably the polarization of the carbonyl carbon being accomplished by the fluorine atoms in the absence of a specific metal ion. That this is not entirely the metal ion function is indicated by the fact that rate enhancements follow the order manganese procarboxypeptidase A approximately zinc procarboxypeptidase greater than apo-procarboxypeptidase. The results indicate considerable similarities for the zymogen-enzyme pair in terms of catalytic groups, pH dependence, specificity and the nature of their transition state binding sites. Some changes in the substrate or inhibitor binding sites are noted.