南非叶及其近缘属的化学指纹图谱：利用高效液相色谱-紫外检测法对南非叶中的氧代孕烷糖苷进行化学分析。
Chemical fingerprinting of Hoodia species and related genera: chemical analysis of oxypregnane glycosides using high-performance liquid chromatography with UV detection in Hoodia gordonii.
作者信息
Avula Bharathi, Wang Yan-Hong, Pawar Rahul S, Shukla Yatin J, Khan Ikhlas A
机构信息
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA.
出版信息
J AOAC Int. 2007 Nov-Dec;90(6):1526-31.
Hoodia gordonii, family Asclepiadaceae, is a succulent plant and is traditionally used in southern Africa for its appetite-suppressant properties. A high-performance liquid chromatographic (HPLC) method with UV detection for analysis of 11 oxypregnane glycosides from H. gordonii has been developed. The simultaneous analysis of 11 oxypregnane glycosides was achieved with a Phenomenex (Torrance, CA) reversed-phase C18 column using gradient mobile phase of water and acetonitrile, both containing 0.025% trifluoroacetic acid. The developed method was applied to the identification of oxypregnane glycosides in 3 different species of Hoodia and 23 related genera. The HPLC profiles of various plant samples were compared for the presence of oxypregnane glycosides.
南非沙漠瓜，属于萝藦科，是一种多肉植物，在非洲南部传统上因其具有抑制食欲的特性而被使用。已开发出一种采用紫外检测的高效液相色谱（HPLC）方法，用于分析南非沙漠瓜中的11种氧代孕烷糖苷。使用菲罗门（加利福尼亚州托兰斯）反相C18柱，以水和乙腈作为梯度流动相（二者均含有0.025%的三氟乙酸），实现了对11种氧代孕烷糖苷的同时分析。所开发的方法被应用于鉴定3种不同的南非沙漠瓜属植物及23个相关属中的氧代孕烷糖苷。比较了各种植物样品的HPLC图谱，以确定氧代孕烷糖苷的存在情况。
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