Planck Stephen R, Becker Matthias D, Crespo Sergio, Choi Dongseok, Galster Kellen, Garman Kiera L, Nobiling Rainer, Rosenbaum James T
Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
Inflammation. 2008 Apr;31(2):105-11. doi: 10.1007/s10753-007-9055-x. Epub 2008 Jan 15.
Extravascular neutrophil migration is poorly characterized in vivo. To test the hypothesis that this migration is a non-random process, we used videomicroscopy to monitor neutrophils in irises of living mice with endotoxin-induced uveitis (EIU). Paths of individual cells were analyzed. Nearly all of these cells were moving in divergent directions, and mean displacement plots indicated that the predominant movement was random. The paths of some cells were fit to bivariate autoregressive integrated moving average models that revealed at least two modes of movement: random search and linear trend. Cell speed was significantly reduced by the actin inhibitor, cytochalasin D. The pattern of migration for neutrophils is in marked contrast to what we previously described for antigen-presenting cells in the iris, but somewhat resembles recent descriptions for T cells within a lymph node. Characterization of extravascular migration of neutrophils has important implications for understanding infection and immunity.
血管外中性粒细胞迁移在体内的特征尚不明确。为了验证这种迁移是一个非随机过程的假设，我们使用视频显微镜来监测患有内毒素诱导性葡萄膜炎（EIU）的活体小鼠虹膜中的中性粒细胞。分析了单个细胞的运动轨迹。几乎所有这些细胞都朝着不同方向移动，平均位移图表明主要运动是随机的。一些细胞的轨迹符合双变量自回归积分移动平均模型，该模型揭示了至少两种运动模式：随机搜索和线性趋势。肌动蛋白抑制剂细胞松弛素D显著降低了细胞速度。中性粒细胞的迁移模式与我们之前描述的虹膜中抗原呈递细胞的迁移模式形成鲜明对比，但在一定程度上类似于最近对淋巴结内T细胞的描述。中性粒细胞血管外迁移的特征对于理解感染和免疫具有重要意义。
