Klećkowska-Nawrot J, Dziegiel P
Department of Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Wrocław, ul. Kozuchowska 1/3, 51-631 Wrocław, Poland.
Anat Histol Embryol. 2008 Feb;37(1):36-40. doi: 10.1111/j.1439-0264.2007.00794.x.
The morphological and histological examinations of the deep gland of the third eyelid were carried out on pig foetuses coming from the 35th, 50th, 63rd, 94th and 112th day of gestation. The morphological examinations were conducted using the method of macroscopic preparation with a forehead magnifying glass and binocular (magnification 1.5-5.0x). In order to make anatomical elements more visible, 60-80% absolute alcohol and 0.5-4% acetic acid solution were used for the examinations. For the histological examinations, the whole eyeball with developing accessory organs was collected from the pig foetuses on the 35th day of gestation. On the 50th, 63rd, 94th and 112th day of gestation only the deep gland of the third eyelid was collected. Staining with haematoxylin-eosin and Azan method was performed. It was found during the examinations that the process of the formation of the deep gland of the third eyelid starts on the 35th day of gestation. On the 50th day of gestation, the gland cells are evenly distributed in the connective tissue stroma. On the 63rd day of gestation, the connective tissue divides the gland parenchyma into indistinct lobes composed of 6-15 lobules. On the 94th day of gestation, the gland lobes become visible; the efferent ducts are situated in the central part of the lobe. On the 112th day of gestation, the lobes are composed of a high number of lobules composed of two kinds of excretory ducts. The first type of the excretory ducts is lined with the simple cuboid epithelium whose nuclei are situated at the base of the cell. The other type of the excretory ducts is lined with the simple cuboid epithelium whose nuclei are round and arranged less or more peripherally.
对妊娠第35、50、63、94和112天的猪胎儿第三眼睑深腺进行了形态学和组织学检查。形态学检查采用前额放大镜和双目显微镜（放大倍数1.5 - 5.0倍）进行宏观标本制备的方法。为使解剖结构更清晰可见，检查时使用了60 - 80%的无水乙醇和0.5 - 4%的醋酸溶液。组织学检查方面，在妊娠第35天从猪胎儿收集带有发育中附属器官的整个眼球。在妊娠第50、63、94和112天仅收集第三眼睑深腺。进行了苏木精 - 伊红染色和阿赞染色法。检查发现，第三眼睑深腺的形成过程始于妊娠第35天。妊娠第50天，腺细胞均匀分布于结缔组织基质中。妊娠第63天，结缔组织将腺实质分成由6 - 15个小叶组成的不明显叶。妊娠第94天，腺叶可见；输出管位于叶的中央部分。妊娠第112天，叶由大量由两种排泄管组成的小叶构成。第一种排泄管内衬单层立方上皮，其细胞核位于细胞基部。另一种排泄管内衬单层立方上皮，其细胞核呈圆形，或多或少排列在周边。
