Craig Fiona E, Foon Kenneth A
Division of Hematopathology, Department of Pathology, University of Pittsburgh School of Medicine, PA, USA.
Blood. 2008 Apr 15;111(8):3941-67. doi: 10.1182/blood-2007-11-120535. Epub 2008 Jan 15.
Flow cytometric immunophenotyping remains an indispensable tool for the diagnosis, classification, staging, and monitoring of hematologic neoplasms. The last 10 years have seen advances in flow cytometry instrumentation and availability of an expanded range of antibodies and fluorochromes that have improved our ability to identify different normal cell populations and recognize phenotypic aberrancies, even when present in a small proportion of the cells analyzed. Phenotypically abnormal populations have been documented in many hematologic neoplasms, including lymphoma, chronic lymphoid leukemias, plasma cell neoplasms, acute leukemia, paroxysmal nocturnal hemoglobinuria, mast cell disease, myelodysplastic syndromes, and myeloproliferative disorders. The past decade has also seen refinement of the criteria used to identify distinct disease entities with widespread adoption of the 2001 World Health Organization (WHO) classification. This classification endorses a multiparametric approach to diagnosis and outlines the morphologic, immunophenotypic, and genotypic features characteristic of each disease entity. When should flow cytometric immunophenotyping be applied? The recent Bethesda International Consensus Conference on flow cytometric immunophenotypic analysis of hematolymphoid neoplasms made recommendations on the medical indications for flow cytometric testing. This review discusses how flow cytometric testing is currently applied in these clinical situations and how the information obtained can be used to direct other testing.
流式细胞术免疫表型分析仍然是血液系统肿瘤诊断、分类、分期及监测不可或缺的工具。在过去十年中，流式细胞术仪器取得了进展，并且有了更多种类的抗体和荧光染料，这提高了我们识别不同正常细胞群体以及识别表型异常的能力，即使这些异常存在于所分析细胞的一小部分中。在许多血液系统肿瘤中都记录到了表型异常群体，包括淋巴瘤、慢性淋巴细胞白血病、浆细胞肿瘤、急性白血病、阵发性睡眠性血红蛋白尿、肥大细胞疾病、骨髓增生异常综合征和骨髓增殖性疾病。在过去十年中，随着2001年世界卫生组织（WHO）分类的广泛采用，用于识别不同疾病实体的标准也得到了完善。该分类认可多参数诊断方法，并概述了每个疾病实体的形态学、免疫表型和基因型特征。何时应进行流式细胞术免疫表型分析？最近关于血液淋巴系统肿瘤流式细胞术免疫表型分析的贝塞斯达国际共识会议对流式细胞术检测的医学指征提出了建议。本综述讨论了流式细胞术检测目前在这些临床情况下的应用方式，以及所获得的信息如何用于指导其他检测。
