Takabatake Yoshitsugu, Isaka Yoshitaka, Imai Enyu
Department of Nephrology, Osaka University Graduate School of Medicine, Osaka, Japan.
Nephrology (Carlton). 2008 Feb;13(1):23-6. doi: 10.1111/j.1440-1797.2007.00907.x.
We investigated whether injection of synthetic small interfering siRNAs via renal artery followed by electroporation could be effective in silencing specific genes in glomerulus.
Solution of siRNA targeting enhanced green fluorescent protein (EGFP) was injected via the renal artery of EGFP-transgenic rats followed by delivery of electric pulses.
The delivery of siRNA reduced endogenous EGFP expression, mainly in glomerular mesangial cells.
Electroporation-mediated gene transfer system via renal artery in rats is very useful for the delivery of a range of different materials including cells, viruses and proteins. Here, we provide a step-by-step description of this perfusion method and a discussion of the key points for the success of the technique.
我们研究了经肾动脉注射合成的小干扰RNA（siRNA）并随后进行电穿孔是否能有效沉默肾小球中的特定基因。
将靶向增强型绿色荧光蛋白（EGFP）的siRNA溶液经肾动脉注射到EGFP转基因大鼠体内，随后施加电脉冲。
siRNA的递送降低了内源性EGFP的表达，主要是在肾小球系膜细胞中。
大鼠经肾动脉的电穿孔介导的基因转移系统对于递送包括细胞、病毒和蛋白质在内的一系列不同物质非常有用。在此，我们提供了这种灌注方法的详细步骤描述以及该技术成功的关键点讨论。
