Cortés-Castillo M A, Favila-Castillo L
Departamento de Inmunologia, Escuela Nacional de Ciencias Biológicas, México, D.F.
Arch Invest Med (Mex). 1991 Jan-Mar;22(1):35-40.
We prepared supernatants of Concanavalin-A activated human lymphocytes containing high titers of leukocyte migration inhibition factor (LIF). A pool of these supernatants was filtered thorough sephadex 6-100 as well as a pool of supernatants from parallel non activated cultures. A migration assay was carried out for each activated fraction, using as control migration the same fraction from non activated supernatants. In this way we found a fraction from activated supernatants with high LIF activity. We assayed the effect of this LIF containing fraction on a yeast endocytosis assay by polymorphonuclear (PMN) cells. We found that the LIF containing fraction increased the number of endocytic PMN in about 80%. This effect was absent from control supernatant and from other fractions from activated supernatant but without LIF activity. The LIF containing fraction did not increase the average number of endocytosed yeast per cell nor the ability to reduce NBT. The endocytosis enhancing effect was blocked by the specific LIF blocker N-acetyl-D-glucosamine. We conclude that LIF can increase the endocytic activity of PMN cells.
我们制备了含有高滴度白细胞迁移抑制因子（LIF）的伴刀豆球蛋白A激活的人淋巴细胞的上清液。将这些上清液的一个池通过葡聚糖凝胶6 - 100进行过滤，同时对来自平行未激活培养物的上清液池也进行过滤。对每个激活部分进行迁移试验，以未激活上清液中的相同部分作为对照迁移。通过这种方式，我们从激活的上清液中找到了具有高LIF活性的一个部分。我们通过多形核（PMN）细胞的酵母内吞试验检测了这个含LIF部分的作用。我们发现含LIF部分使内吞PMN的数量增加了约80%。对照上清液以及来自激活上清液但无LIF活性的其他部分均无此作用。含LIF部分既未增加每个细胞内吞酵母的平均数量，也未增加其还原NBT的能力。内吞增强作用被特异性LIF阻断剂N - 乙酰 - D - 葡萄糖胺所阻断。我们得出结论，LIF可增加PMN细胞的内吞活性。
