Villarreal M L, Muñoz J
Unidad de Investigación en Medicina Tradicional y Desarrollo de Medicamentos, I.M.S.S., Morelos, México.
Arch Invest Med (Mex). 1991 Apr-Jun;22(2):127-33.
A tissue culture method is described for micropropagation and callus formation from Solanum chrysotricum axillary bud explants in Murashige and Skoog's (MS) medium, supplemented with various growth regulators. Induction of rooted plants were initiated only when indol-3 acetic acid (IAA) was present as an auxin in combination with either of two cytokinins: kinetin (KN) or benzyladenine (BA); however, the combination of IAA (0.1 mg.lt.-1) + BA (0.2 mg.lt.-1) was found to be best suited for morphogenesis purposes. Alternatively, callus tissue formation was influenced in presence of naphthalene acetic acid; which in combination with kinetin (NAA 0.1 mg.lt.-1 + KN 0.2 mg.lt.-1) exhibit the best response studied. The plant material obtained by this procedure is proposed for pharmacological and chemical studies of this important antimycotic plant remedy.
本文描述了一种组织培养方法，用于在添加了各种生长调节剂的Murashige和Skoog（MS）培养基中，以金灯果腋芽外植体进行微繁殖和愈伤组织形成。仅当吲哚 - 3 - 乙酸（IAA）作为生长素与两种细胞分裂素之一：激动素（KN）或苄基腺嘌呤（BA）结合存在时，才开始诱导生根植株；然而，发现IAA（0.1 mg.l⁻¹）+ BA（0.2 mg.l⁻¹）的组合最适合形态发生目的。另外，萘乙酸的存在会影响愈伤组织的形成；其中与激动素组合（萘乙酸0.1 mg.l⁻¹ + 激动素0.2 mg.l⁻¹）表现出所研究的最佳反应。通过该程序获得的植物材料被提议用于这种重要抗真菌植物药物的药理和化学研究。
