用于木瓜蛋白酶催化肽合成的木瓜蛋白酶在环氧聚合物上的固定化。

Immobilization of papain on epoxy-polymers for papain-catalyzed peptide synthesis.

作者信息

Eckstein H, Renner H J, Brun H

机构信息

Institute of Organic Chemistry, University of Tübingen, FRG.

出版信息

Biomed Biochim Acta. 1991;50(10-11):S114-7.

PMID:1820030

Abstract

In this paper we describe the immobilization of papain on two commercially available carriers, VA Biosynth and Eupergit C, both in their epoxidized form. Papain can be immobilized on both polymers and shows high activity. The stability (lyophilization, storage) of the papain-VA Biosynth is very good. Even after prolonged storage (-20 degrees C) it can be reactivated to its full activity.

摘要

在本文中，我们描述了木瓜蛋白酶固定在两种市售载体VA Biosynth和Eupergit C上的情况，这两种载体均为环氧化形式。木瓜蛋白酶可以固定在这两种聚合物上，并表现出高活性。木瓜蛋白酶-VA Biosynth的稳定性（冻干、储存）非常好。即使经过长时间储存（-20摄氏度），它仍可重新激活至其全部活性。