Voyushina T L, Stepanov V M
Institute for Genetics and Selection of Industrial Microorganisms, Moscow, USSR.
Biomed Biochim Acta. 1991;50(10-11):S209-12.
The synthesis of the chromogenic substrates for trypsin-like proteinases catalyzed by alpha-chymotrypsin and subtilisin from B. subtilis strain 72 were carried out in the organic media at a low water content using the enzymes adsorbed on different porous materials. The method proposed allows us to vary the structure of the compounds to be synthesized and is a suitable technique for their scaling-up.
使用吸附在不同多孔材料上的酶，在低含水量的有机介质中，由α-胰凝乳蛋白酶和枯草芽孢杆菌72株的枯草杆菌蛋白酶催化合成类胰蛋白酶的生色底物。所提出的方法使我们能够改变待合成化合物的结构，并且是一种适合扩大其规模的技术。
