van Liempt H, Pfeifer E, Schwecke T, Palissa H, von Doehren H
Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, F.R.G.
Biomed Biochim Acta. 1991;50(10-11):S256-9.
The amino acid sequences of the genes coding for four multienzyme peptide synthetases, operating by the thiotemplate mechanism are compared, to show underlying principles in the biosynthetic mechanism. Alignment with other carboxylic acid activating enzymes shows the sequences. LAY(V/I)I(Y/F)TSGT(T/S)GxPKGV and GELx(L/I)GGxG(V/I) to be involved in MgATP2-binding and adenylate formation, and two other sequences, one containing the element FxLGG(H/D)S(I/L) to be involved in covalent binding of the amino acid. As a general rule, 1000 amino acid building blocks are responsible for the incorporation of one amino acid into the nascent peptide.
对四种通过硫酯模板机制起作用的多酶肽合成酶的编码基因的氨基酸序列进行了比较，以揭示生物合成机制中的潜在原理。与其他羧酸活化酶的比对显示了这些序列。LAY(V/I)I(Y/F)TSGT(T/S)GxPKGV和GELx(L/I)GGxG(V/I)参与MgATP2结合和腺苷酸形成，另外两个序列，其中一个包含FxLGG(H/D)S(I/L)元件，参与氨基酸的共价结合。一般来说，1000个氨基酸构建块负责将一个氨基酸掺入新生肽中。
