Bemquerer M P, Theobaldo F C, Tominaga M
Department of Biochemistry, University of Sao Paulo, Brazil.
Biomed Biochim Acta. 1991;50(10-11):S94-7.
Pepsin catalyzed peptide synthesis in biphasic systems containing more than 95% (v/v) of organic phase was studied. Good yields were only obtained with more hydrophobic solvents, in spite of the low solubility of the substrates. The effects of the following parameters were also investigated: concentration of amino and carboxylic components, pH and buffer concentration, ratio between the aqueous and organic phases. The influence of different amino acid residues in the P2 position was investigated through the coupling between Z-Xyz-Phe-OH (where Xyz = Ala, Phe, Trp and Tyr) and Phe-OMe.
研究了胃蛋白酶在有机相含量超过95%（v/v）的双相体系中催化的肽合成反应。尽管底物的溶解度较低，但只有使用疏水性更强的溶剂才能获得较高的产率。还研究了以下参数的影响：氨基和羧基组分的浓度、pH值和缓冲液浓度、水相和有机相之间的比例。通过Z-Xyz-Phe-OH（其中Xyz = Ala、Phe、Trp和Tyr）与Phe-OMe的偶联反应，研究了P2位置不同氨基酸残基的影响。
