Marsili Enrico, Beyenal Haluk, Di Palma Luca, Merli Carlo, Dohnalkova Alice, Amonette James E, Lewandowski Zbigniew
Center for Biofilm Engineering, Department of Civil Engineering, Montana State University, Bozeman, Montana 59717, USA.
Environ Sci Technol. 2007 Dec 15;41(24):8349-54. doi: 10.1021/es071335k.
Biofilms of sulfate-reducing bacteria Desulfovibrio desulfuricans G20 were used to reduce dissolved U(VI) and subsequently immobilize U(IV) in the presence of uranium-complexing carbonates. The biofilms were grown in three identically operated fixed bed reactors, filled with three types of minerals: one noncarbonate-bearing mineral (hematite) and two carbonate-bearing minerals (calcite and dolomite). The source of carbonates in the reactors filled with calcite and dolomite were the minerals, while in the reactor filled with hematite it was a 10 mM carbonate buffer, pH 7.2, which we added to the growth medium. Our five-month study demonstrated that the sulfate-reducing biofilms grown in all reactors were able to immobilize/reduce uranium efficiently, despite the presence of uranium-complexing carbonates.
利用硫酸盐还原菌脱硫脱硫弧菌G20的生物膜在铀络合碳酸盐存在的情况下还原溶解态U(VI)，随后固定U(IV)。生物膜在三个操作相同的固定床反应器中生长，这些反应器填充了三种类型的矿物：一种不含碳酸盐的矿物（赤铁矿）和两种含碳酸盐的矿物（方解石和白云石）。填充方解石和白云石的反应器中碳酸盐的来源是矿物，而填充赤铁矿的反应器中碳酸盐的来源是我们添加到生长培养基中的10 mM碳酸盐缓冲液（pH 7.2）。我们为期五个月的研究表明，尽管存在铀络合碳酸盐，但在所有反应器中生长的硫酸盐还原生物膜都能够有效地固定/还原铀。
