Shintani Yoichi, Yasuda Yoko, Kobayashi Keiko, Maeda Akira, Morita Akimichi
Department of Geriatric and Environmental Dermatology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Photodermatol Photoimmunol Photomed. 2008 Feb;24(1):32-7. doi: 10.1111/j.1600-0781.2008.00333.x.
BACKGROUND/PURPOSE: A main mechanism responsible for the efficacy of narrowband ultraviolet (UV)B is thought to be the induction of apoptosis in pathogenetically relevant cells. Narrowband UVB therapy, however, generally induces a relatively long remission period. Recently, evidence that UVB radiation induces regulatory T (Treg) cells was reported. Based on these findings, we examined whether narrowband UVB suppresses contact hypersensitivity (CHS) by inducing Treg cells.
The shaved abdomens of C3H/HeN mice were irradiated with broadband or narrowband UVB. CHS was defined as an ear-swelling response. To examine whether tolerance can be induced by adoptive transfer, lymph node cells from UVB-irradiated mice were injected into naïve mice before sensitization and CHS challenge.
Narrowband UVB exposure dose dependently suppressed CHS. Significant suppression was observed at doses between 1000 and 3000 mJ/cm(2) (P<0.05). The suppressive effect achieved with 1000 mJ/cm(2) narrowband UVB was very similar to the effect achieved with 100 mJ/cm(2) broadband UVB. The suppressive effects on CHS were transferred to naïve mice by the injection of lymph node cells from tolerant mice.
Narrowband UVB induced local and systemic suppression of CHS. In addition, narrowband UVB induces tolerance to CHS and the suppressive effects were transferable to naïve mice.
背景/目的：窄谱中波紫外线（UV）B发挥疗效的主要机制被认为是诱导致病相关细胞发生凋亡。然而，窄谱UVB疗法通常会诱导相对较长的缓解期。最近，有报道称UVB辐射可诱导调节性T（Treg）细胞。基于这些发现，我们研究了窄谱UVB是否通过诱导Treg细胞来抑制接触性超敏反应（CHS）。
对C3H/HeN小鼠剃毛后的腹部进行宽谱或窄谱UVB照射。CHS定义为耳部肿胀反应。为了研究通过过继转移是否可诱导耐受性，在致敏和CHS激发前，将来自UVB照射小鼠的淋巴结细胞注射到未致敏小鼠体内。
窄谱UVB照射剂量依赖性地抑制CHS。在1000至3000 mJ/cm²的剂量下观察到显著抑制（P<0.05）。1000 mJ/cm²窄谱UVB所产生的抑制效果与100 mJ/cm²宽谱UVB所产生的效果非常相似。通过注射来自耐受小鼠的淋巴结细胞，对CHS的抑制作用可转移至未致敏小鼠。
窄谱UVB诱导CHS的局部和全身抑制。此外，窄谱UVB诱导对CHS的耐受性，且抑制作用可转移至未致敏小鼠。
