Lavie F, Miceli-Richard C, Ittah M, Sellam J, Gottenberg J-E, Mariette X
Rheumatology Department, Hôpital Bicêtre, Assistance Publique-Hôpitaux de Paris, Institut Pour Santé et Recherche Médicale (INSERM) U802, Université Paris-Sud 11, Le Kremlin Bicêtre, France.
Scand J Immunol. 2008 Feb;67(2):185-92. doi: 10.1111/j.1365-3083.2007.02049.x.
We investigated B-cell activating factor of the tumour necrosis factor family (BAFF) level in peripheral blood mononuclear cells (PBMCs), monocytes and T cells from patients with primary Sjögren's syndrome (pSS) and controls both ex vivo and in vitro after cytokine stimulation. PBMCs, monocytes and T cells were isolated from 15 patients with pSS and 17 controls. Cells were cultured alone or with interferon (IFN)alpha, IFNgamma and interleukin 10 (IL-10). T cells were stimulated with phytohaemagglutin and anti-CD3. BAFF protein was assessed by enzyme-linked immunosorbent assay. Ex vivo, no difference was observed in BAFF mRNA level in PBMCs and monocytes from patients and controls. Blood monocytes were the main cell type secreting BAFF both in patients and controls. In vitro, after IFNalpha stimulation, BAFF mRNA level was significantly higher in cells from patients than from controls (63.8 versus 20.7, P = 0.03). T cells from patients secreted a higher level of BAFF protein than those from healthy donor cells (17.4 versus 2.9 pg/ml, respectively, P = 0.04) but at a lower level than that from monocytes. Stimulation of T cells did not change BAFF secretion level. The induction of Th17 cells showed no increased BAFF expression. In conclusion, similar to epithelial cells, blood monocytes in patients with pSS show increased production of BAFF under IFNalpha, which confirms the involvement of IFNalpha in pSS. BAFF expression is also increased in blood T cells of such patients, independently of T-cell stimulation.
我们研究了原发性干燥综合征（pSS）患者及对照外周血单个核细胞（PBMC）、单核细胞和T细胞中肿瘤坏死因子家族B细胞活化因子（BAFF）水平，包括体外及细胞因子刺激后的体外水平。从15例pSS患者和17名对照中分离出PBMC、单核细胞和T细胞。细胞单独培养或与干扰素（IFN）α、IFNγ和白细胞介素10（IL-10）一起培养。用植物血凝素和抗CD3刺激T细胞。通过酶联免疫吸附测定评估BAFF蛋白。在体外，患者和对照的PBMC及单核细胞中BAFF mRNA水平未观察到差异。血液单核细胞是患者和对照中分泌BAFF的主要细胞类型。在体外，IFNα刺激后，患者细胞中的BAFF mRNA水平显著高于对照（分别为63.8对20.7，P = 0.03）。患者的T细胞分泌的BAFF蛋白水平高于健康供体细胞（分别为17.4对2.9 pg/ml，P = 0.04），但低于单核细胞。T细胞刺激未改变BAFF分泌水平。Th17细胞的诱导未显示BAFF表达增加。总之，与上皮细胞类似，pSS患者的血液单核细胞在IFNα作用下BAFF产生增加，这证实了IFNα参与pSS。此类患者血液T细胞中的BAFF表达也增加，与T细胞刺激无关。
