Roveta S, Marchese A, Schito G C
Sezione di Microbiologia, Di.S.C.A.T., University of Genoa, Largo R. Benzi 10, 16132 Genoa, Italy.
Int J Antimicrob Agents. 2008 Apr;31(4):321-8. doi: 10.1016/j.ijantimicag.2007.11.012. Epub 2008 Feb 21.
The aim of this study was to assess whether the novel lipopeptide daptomycin might be capable of disrupting or inhibiting the synthesis of biofilms produced by staphylococci. Fourteen recently isolated slime-producing methicillin-susceptible (MET-S) and methicillin-resistant (MET-R) strains (three MET-S Staphylococcus aureus, three MET-R S. aureus, three MET-S Staphylococcus epidermidis, three MET-R S. epidermidis and two vancomycin-intermediate S. aureus (VISA)) were tested. Slime formation on polystyrene plates was quantified spectrophotometrically. Daptomycin (2-64 mg/L) inhibited slime synthesis by > or =80% in MET-S strains, by 60-80% in MET-R S. aureus and by 70-95% in MET-R S. epidermidis. At 64 mg/L, biofilm synthesis decreased by 80% in the VISA isolates. Daptomycin also disrupted pre-formed biofilm: >50% breakdown of initial biofilm (5h) was observed in all strains. Disruption of mature biofilms (48 h), in terms of percentage, was more variable depending on the strain, ranging from ca. 20% in a MET-R S. epidermidis strain to almost 70% in two MET-S strains (one S. aureus and one S. epidermidis). Daptomycin at concentrations achievable during therapy promoted a statistically significant inhibition of slime synthesis (preventing biofilm building) and induced slime disruption (disaggregating its structure) both in initial and mature biofilms on a plastic support in all staphylococcal strains studied.
本研究的目的是评估新型脂肽达托霉素是否能够破坏或抑制葡萄球菌产生的生物膜的合成。对14株最近分离出的产黏液的甲氧西林敏感（MET-S）和甲氧西林耐药（MET-R）菌株（3株MET-S金黄色葡萄球菌、3株MET-R金黄色葡萄球菌、3株MET-S表皮葡萄球菌、3株MET-R表皮葡萄球菌和2株万古霉素中介金黄色葡萄球菌（VISA））进行了测试。通过分光光度法定量测定聚苯乙烯平板上的黏液形成情况。达托霉素（2 - 64 mg/L）在MET-S菌株中抑制黏液合成>或 = 80%，在MET-R金黄色葡萄球菌中抑制60 - 80%，在MET-R表皮葡萄球菌中抑制70 - 95%。在64 mg/L时，VISA分离株中的生物膜合成减少了80%。达托霉素还能破坏预先形成的生物膜：在所有菌株中均观察到初始生物膜（5小时）有>50%的分解。就百分比而言，成熟生物膜（48小时）的破坏情况因菌株而异，范围从MET-R表皮葡萄球菌菌株中的约20%到两株MET-S菌株（一株金黄色葡萄球菌和一株表皮葡萄球菌）中的近70%。在本研究的所有葡萄球菌菌株中，治疗期间可达到的浓度的达托霉素在塑料载体上对初始和成熟生物膜均促进了黏液合成的统计学显著抑制（防止生物膜形成）并诱导了黏液破坏（分解其结构）。
