Ogawa Mari, Shinohara Hidefumi, Sakagami Youji, Matsubayashi Yoshikatsu
Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Science. 2008 Jan 18;319(5861):294. doi: 10.1126/science.1150083.
CLV1, which encodes a leucine-rich repeat receptor kinase, and CLV3, which encodes a secreted peptide, function in the same genetic pathway to maintain stem cell populations in Arabidopsis shoot apical meristem. Here, we show biochemical evidence, by ligand binding assay and photoaffinity labeling, that the CLV3 peptide directly binds the CLV1 ectodomain with a dissociation constant of 17.5 nM. The CLV1 ectodomain also interacts with the structurally related CLE peptides, with distinct affinities depending on the specific amino acid sequence. Our results provide direct evidence that CLV3 and CLV1 function as a ligand-receptor pair involved in stem cell maintenance.
CLV1编码一种富含亮氨酸重复序列的受体激酶，CLV3编码一种分泌肽，它们在同一遗传途径中发挥作用，以维持拟南芥茎尖分生组织中的干细胞群体。在这里，我们通过配体结合试验和光亲和标记展示了生化证据，即CLV3肽以17.5 nM的解离常数直接结合CLV1胞外域。CLV1胞外域也与结构相关的CLE肽相互作用，根据特定氨基酸序列具有不同的亲和力。我们的结果提供了直接证据，表明CLV3和CLV1作为参与干细胞维持的配体-受体对发挥作用。
