Hennig A, Fink-Gremmels J, Leistner L
Federal Institute for Meat Research, Kulmbach, Germany.
IARC Sci Publ. 1991(115):255-60.
Primary cultures of hepatocytes derived from untreated rats were incubated in the presence of ochratoxin A for 24 h. Five different strains of histidine auxotroph Salmonella typhimurium were exposed to conditioned cell culture medium before being tested for mutagenicity. A clear hepatocyte-mediated mutagenic response was observed in TA1535, TA1538 and TA100. In addition, sister chromatid exchange frequency was increased in human peripheral lymphocytes that had been incubated in the presence of conditioned medium derived from ochratoxin A-exposed hepatocytes.
将未处理大鼠的肝细胞原代培养物在存在赭曲霉毒素A的条件下孵育24小时。在测试致突变性之前，将五种不同的组氨酸营养缺陷型鼠伤寒沙门氏菌菌株暴露于条件细胞培养基中。在TA1535、TA1538和TA100中观察到明显的肝细胞介导的致突变反应。此外，在存在由暴露于赭曲霉毒素A的肝细胞产生的条件培养基中孵育的人外周血淋巴细胞中，姐妹染色单体交换频率增加。
