间接免疫荧光染色法在石蜡切片中检测肠炎沙门氏菌及感染鸭组织中抗原定位的应用。
Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues.
作者信息
Yan Bin, Cheng An-Chun, Wang Ming-Shu, Deng Shu-Xuan, Zhang Zhen-Hua, Yin Nian-Chun, Cao Ping, Cao Sheng-Yan
机构信息
Avian Disease Research Center, Collage of Veterinary Medicine of Sichuan Agriculture University, Yaan 625014, Sichuan Province, China.
出版信息
World J Gastroenterol. 2008 Feb 7;14(5):776-81. doi: 10.3748/wjg.14.776.
AIM
To detect Salmonella enteritidis (S. enteritidis) in paraffin slices and antigen location in infected duck tissues.
METHODS
The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method (IFA) was established to detect the S. enteritidis antigen in paraffin slices. Detected S. enteritidis in each organ tissue of ducklings experimentally infected with S. enteritidis.
RESULTS
The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen mainly distributed in the infected cell cytoplasm.
CONCLUSION
IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis. We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium.
目的
检测石蜡切片中的肠炎沙门氏菌（肠炎杆菌）及感染鸭组织中的抗原定位。
方法
用纯化杆菌免疫家兔以获得肠炎杆菌特异性抗体，然后通过辛酸 - 硫酸铵法提取，经High - Q柱纯化。建立间接免疫荧光染色法（IFA）检测石蜡切片中的肠炎杆菌抗原。检测经肠炎杆菌实验感染的雏鸭各器官组织中的肠炎杆菌。
结果
实验感染肠炎杆菌的雏鸭的哈德氏腺、心脏、肾脏、脾脏、肝脏、脑、回肠、空肠、法氏囊呈阳性或强阳性，肠炎杆菌抗原主要分布在感染细胞的细胞质中。
结论
IFA是检测石蜡切片中肠炎杆菌抗原的直观、灵敏且特异的方法，是肠炎杆菌诊断及抗原定位的良好方法。我们还得出结论，哈德氏腺、心脏、肾脏、脾脏、肝脏、回肠、空肠是鸭肠炎杆菌感染的靶器官，且肠炎杆菌是一种细胞内寄生菌。
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