马铃薯中一个新的CONSTANS类基因的分子克隆与表达分析
Molecular cloning and expression analysis of a novel CONSTANS-like gene from potato.
作者信息
Guo J L, Yang Q, Liang F, Xing Y J, Wang Z
机构信息
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
出版信息
Biochemistry (Mosc). 2007 Nov;72(11):1241-6. doi: 10.1134/s0006297907110107.
A full-length cDNA of a StCONSTANS-like (StCOL) gene was cloned from potato (Solanum tuberosum L.) by RT-PCR and RACE. The predicted amino acid sequence of this cDNA has a high degree of identity with other homologous members of the CO or COL family. Analysis of mRNA levels for StCOL shows that it is highly expressed in leaves and becomes weaker during tuberization; moreover, is independent of gibberellin A(3) and sucrose.
通过逆转录聚合酶链反应（RT-PCR）和cDNA末端快速扩增技术（RACE），从马铃薯（Solanum tuberosum L.）中克隆出一个全长的类CONSTANS基因（StCONSTANS-like，StCOL）。该cDNA预测的氨基酸序列与CO或COL家族的其他同源成员具有高度同源性。对StCOL基因mRNA水平的分析表明，它在叶片中高度表达，在块茎形成过程中表达减弱；此外，其表达不依赖于赤霉素A(3)和蔗糖。
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