Wagner W, Wehrmann M
Department of Otorhinolaryngology, University of Tübingen, Tübingen, Germany.
J Cell Mol Med. 2007 Nov-Dec;11(6):1342-51. doi: 10.1111/j.1582-4934.2007.00037.x.
Wound-healing mechanisms change during transition from prenatal to postnatal stage. Cytokines are known to play a key role in this process. The current study investigated the differential cytokine activity and healing morphology during healing of incisional skin wounds in rats of the ages neonatal (p0), 3 days old (p3) and adult, after six different healing times (2 hrs to 30 days). All seven tested cytokines (Transforming Growth Factor (TGF) alpha, TGFbeta1, -beta2 and -beta3, IGF 1, Platelet Derived Growth Factor A (PDGF A), basic Fibroblast Growth Factor (bFGF) exhibited higher expression in the adult wounds than at the ages p0 and p3. Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30. The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern. The results may encourage the use of neonatal rat skin as a wound-healing model for further studies, instead of the more complicated prenatal animal models. Secondly, the data may recommend inhibition of PDGF A, basic FGF or TGF-beta1 as therapeutic targets in efforts to optimize wound healing in the adult organism.
从产前到产后阶段，伤口愈合机制会发生变化。已知细胞因子在这一过程中起关键作用。本研究调查了新生（p0）、3日龄（p3）和成年大鼠在六种不同愈合时间（2小时至30天）后，切开皮肤伤口愈合过程中的细胞因子活性差异和愈合形态。所有七种测试的细胞因子（转化生长因子（TGF）α、TGFβ1、-β2和-β3、胰岛素样生长因子1、血小板衍生生长因子A（PDGF A）、碱性成纤维细胞生长因子（bFGF））在成年伤口中的表达均高于p0和p3年龄段。表达通常在受伤后12小时至3天达到峰值，在第10天和第30天时不再可检测到。与成年大鼠相比，新生大鼠标本显示出更快的上皮再形成、更少的炎症和瘢痕形成，以及更大程度的原始组织结构恢复，类似于产前愈合模式。这些结果可能会鼓励使用新生大鼠皮肤作为伤口愈合模型进行进一步研究，而不是更复杂的产前动物模型。其次，这些数据可能建议抑制PDGF A、碱性FGF或TGF-β1作为优化成年生物体伤口愈合的治疗靶点。
