Abdel-Sayed Andrew, Binnert Christophe, Lê Kim-Anne, Bortolotti Murielle, Schneiter Philippe, Tappy Luc
Department of Physiology, School of Biology and Medicine, University of Lausanne, 7 rue du Bugnon, Lausanne CH-1005, Switzerland.
Br J Nutr. 2008 Aug;100(2):393-9. doi: 10.1017/S000711450789547X. Epub 2008 Jan 21.
The effects of a 7 d high-fructose diet (HFrD) or control diet on lipid metabolism were studied in a group of six healthy lean males. Plasma NEFA and beta-hydroxybutyrate concentrations, net lipid oxidation (indirect calorimetry) and exogenous lipid oxidation (13CO2 production) were monitored in basal conditions, after lipid loading (olive oil labelled with [13C]triolein) and during a standardised mental stress. Lactate clearance and the metabolic effects of an exogenous lactate infusion were also monitored. The HFrD lowered plasma concentrations of NEFA and beta-hydroxybutyrate as well as lipid oxidation in both basal and after lipid-loading conditions. In addition, the HFrD blunted the increase in plasma NEFA and exogenous lipid oxidation during mental stress. The HFrD also increased basal lactate concentrations by 31.8 %, and lactate production by 53.8 %, while lactate clearance remained unchanged. Lactate infusion lowered plasma NEFA with the control diet, and net lipid oxidation with both the HFrD and control diet. These results indicate that a 7 d HFrD markedly inhibits lipolysis and lipid oxidation. The HFrD also increases lactate production, and the ensuing increased lactate utilisation may contribute to suppress lipid oxidation.
在一组六名健康瘦男性中研究了7天高果糖饮食（HFrD）或对照饮食对脂质代谢的影响。在基础状态、脂质负荷后（用[13C]三油酸甘油酯标记的橄榄油）以及标准化精神应激期间，监测血浆非酯化脂肪酸（NEFA）和β-羟基丁酸酯浓度、净脂质氧化（间接量热法）和外源性脂质氧化（13CO2生成）。还监测了乳酸清除率以及外源性乳酸输注的代谢效应。HFrD降低了基础状态和脂质负荷后血浆NEFA和β-羟基丁酸酯的浓度以及脂质氧化。此外，HFrD减弱了精神应激期间血浆NEFA和外源性脂质氧化的增加。HFrD还使基础乳酸浓度增加了31.8%，乳酸生成增加了53.8%，而乳酸清除率保持不变。乳酸输注在对照饮食时降低了血浆NEFA，在HFrD和对照饮食时均降低了净脂质氧化。这些结果表明，7天的HFrD显著抑制脂肪分解和脂质氧化。HFrD还增加了乳酸生成，随后增加的乳酸利用可能有助于抑制脂质氧化。
