透明质酸盐在中性和碱性溶液中的构象

Conformation of hyaluronate in neutral and alkaline solutions.

作者信息

Mathews M B, Decker L

出版信息

Biochim Biophys Acta. 1977 Jun 23;498(1):259-63. doi: 10.1016/0304-4165(77)90107-6.

Abstract

Increasing the pH of a neutral salt solution of sodium hyaluronate to 12.5 produces a rapid drop in viscosity which is reversible upon restoring the pH to neutrality. Light scattering data showing a decrease in radius of gyration with no change in molecular weight and negative results with chondroitin and other acidic glycosaminoglycans suggest that the conformational change is specific for hyaluronate molecules.

摘要

将透明质酸钠中性盐溶液的pH值提高到12.5会导致粘度迅速下降，而将pH值恢复到中性时，粘度下降是可逆的。光散射数据显示，回转半径减小，分子量不变，而硫酸软骨素和其他酸性糖胺聚糖的结果为阴性，这表明构象变化是透明质酸分子特有的。