Zhang Xue-lan, Ji Wei, Ji Zheng-hua, Ding Yun-fang, Zhu Hong, Yan Yong-dong, Huang Yi-ping, He Ya-xiang, Ye Jian-xin, Ji Xue-qiang
Clinical Laboratory Children's Hospital Affiliated to Soochow University, Suzhou, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2007 Sep;41(5):371-4.
To probe the epidemiological trend of respiratory syncytial virus (RSV) and cellular immunological change of RSV bronchopneumonia among children in Suzhou in the past five years.
10,205 children with acute respiratory tract infection from January 2001 to December 2005 were enrolled into the study. Nasopharyngeal aspirates were obtained from the respiratory tract by aseptic vacuum aspiration. Direct immuno-fluorescence assay was employed to detect seven kinds of virus antigens including RSV antigen. CD3, CD4, CD8, CD19, CD16 and CD56 in peripheral blood mononuclear cells of 30 patients with RSV bronchopneumonia (1.5-24.0 months old group) were analyzed by flow cytometry analysis, and 15 normal infants (1.5-24.0 months old group) were enrolled as control group.
The annual positive rate of RSV was 24.94%, 25.83%, 24.05%, 25.39% and 27.30% respectively from 2001 to 2005. It also found that the peak season for RSV infection was spring or winter (January to March or November to December). The positive rate of RSV was significantly higher in 1-12 months old group than that in > 12 months old group (chi2 = 97.320, P < 0.01), as well as the groups between 1-12 months old (chi2 = 7.804, P < 0.05, the highest positive rate was occurred at 3-6 months old group). The positive rate of RSV was significantly higher in boys than that in girls (chi2 = 9.693, P < 0.01). The percentages of CD3+, CD4+, CD8+ and NK (CD16 + 56)+ cells were significantly lower in RSV bronchopneumonia than those in control group (t = 3.199, P < 0.01; t = 2.215, P < 0.05; t = 2.619, P < 0.05 and t = 5.240, P < 0.01, respectively). While the percentage of CD19+ cells was significantly elevated in RSV bronchopneumonia than that in control group (t = 2.875, P < 0.01).
RSV infection is of obvious seasonal changes. The younger the patient, the higher positive rates of RSV infection is, while and the cellular immunity function is lower. The effective measures for preventing RSV infection are important, especially for the infants. Further investigation is necessary to understand the causes of the variations for RSV infections between boys and girls.
探讨苏州地区近五年儿童呼吸道合胞病毒（RSV）的流行病学趋势及RSV肺炎患儿的细胞免疫变化。
选取2001年1月至2005年12月期间10205例急性呼吸道感染患儿纳入研究。采用无菌真空抽吸法从呼吸道获取鼻咽抽吸物。采用直接免疫荧光法检测包括RSV抗原在内的七种病毒抗原。对30例RSV肺炎患儿（1.5 - 24.0月龄组）外周血单个核细胞中的CD3、CD4、CD8、CD19、CD16和CD56进行流式细胞术分析，并选取15例正常婴儿（1.5 - 24.0月龄组）作为对照组。
2001年至2005年RSV年阳性率分别为24.94%、25.83%、24.05%、25.39%和27.30%。还发现RSV感染的高峰季节为春季或冬季（1月至3月或11月至12月）。1 - 12月龄组RSV阳性率显著高于>12月龄组（χ2 = 97.320，P < 0.01），1 - 12月龄各亚组间也有差异（χ2 = 7.804，P < 0.05，最高阳性率出现在3 - 6月龄组）。男孩RSV阳性率显著高于女孩（χ2 = 9.693，P < 0.01）。RSV肺炎患儿CD3 +、CD4 +、CD8 +和NK（CD16 + 56）+细胞百分比显著低于对照组（t分别为3.199，P < 0.01；t为2.215，P < 0.05；t为2.619，P < 0.05；t为5.240，P < 0.01）。而RSV肺炎患儿CD19 +细胞百分比显著高于对照组（t = 2.875，P < 0.01）。
RSV感染有明显的季节性变化。患者年龄越小，RSV感染阳性率越高，而细胞免疫功能越低。预防RSV感染的有效措施很重要，尤其是对婴儿。有必要进一步研究了解男孩和女孩RSV感染差异的原因。
