Ganesh K Sankar, Baskaran L, Rajasekaran S, Sumathi K, Chidambaram A L A, Sundaramoorthy P
Department of Botany, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2008 Jun 1;63(2):159-63. doi: 10.1016/j.colsurfb.2007.11.016. Epub 2007 Dec 4.
Water is seriously polluted by the discharge of various industrial wastewater containing heavy metals. Among them, chromium is considered to be toxic to living organisms and it is released mostly from tanneries. The chromium-contaminated water is discharged into nearby water bodies and it affects both aquatic and terrestrial plants. So the present experiment was conducted with an aquatic plant, water lettuce (Pistia stratiotes L.) and a terrestrial plant soybean (Glycine max L. Merr.). They were treated with different concentrations (0, 5, 10, 25, 50, 100 and 200mg/L) of potassium dichromate solution. The biochemical parameters such as total chlorophyll, carotenoid, protein and amino acid content and the enzymatic activities like catalase and peroxidase were estimated. The accumulation of chromium was also analysed in both the plants. All the biochemical contents and enzyme activities of water lettuce and soybean seedlings showed a great variation with respect to the increase in chromium concentrations. The accumulation of chromium increased gradually with the increase of chromium concentrations. Total inhibition of all the parameters were observed at 300 mg/L chromium concentration. The terrestrial plant soybean was sensitive than the aquatic plant water lettuce towards chromium stress.
各种含有重金属的工业废水排放导致水受到严重污染。其中，铬被认为对生物体有毒，且大多来自制革厂。受铬污染的水被排放到附近水体中，影响水生植物和陆生植物。因此，本实验选用了一种水生植物水浮莲（大薸）和一种陆生植物大豆（大豆）进行。它们用不同浓度（0、5、10、25、50、100和200mg/L）的重铬酸钾溶液处理。测定了总叶绿素、类胡萝卜素、蛋白质和氨基酸含量等生化参数以及过氧化氢酶和过氧化物酶等酶活性。还分析了两种植物中铬的积累情况。随着铬浓度的增加，水浮莲和大豆幼苗的所有生化含量和酶活性都有很大变化。铬的积累随着铬浓度的增加而逐渐增加。在铬浓度为300mg/L时，所有参数均出现完全抑制。陆生植物大豆对铬胁迫比水生植物水浮莲更敏感。
