Matsuda F, Hayashi T, Naruse H
National Center for Nervous, Mental and Muscular Disorders, Tokai University School of Medicine, Kanagawa, Japan.
Tokai J Exp Clin Med. 1991 Dec;16(5-6):245-52.
A sensitive gas chromatography/negative ion chemical ionization mass spectrometric (GC/NICIMS) method was devised and by using deuterated L-tryptophan-3,3-d2(Trp-d2) as a tracer, the detailed in vivo metabolism of serotonin (5-HT) and tryptamine (TA) was investigated. A human was administered orally with 10 mg/kg Trp-d2 and rats were injected intra-peritoneally with 50 mg/kg Trp-d2. The ratios of the level of 5-HT-d2 derived from Trp-d2 to that of endogenous 5-HT-d0 and that of TA-d2 to that of TA-d0 were measured in urine and some organs of rats at times up to 4 hr after administration. It is concluded that urinary excretion of 5-HT might reflect its turnover in the central nervous system and that of TA might reflect its peripheral turnover.
设计了一种灵敏的气相色谱/负离子化学电离质谱法（GC/NICIMS），并以氘代L-色氨酸-3,3-d2（Trp-d2）作为示踪剂，研究了血清素（5-HT）和色胺（TA）详细的体内代谢情况。给一名人类口服10 mg/kg的Trp-d2，给大鼠腹腔注射50 mg/kg的Trp-d2。在给药后长达4小时的时间里，测量了大鼠尿液和一些器官中源自Trp-d2的5-HT-d2水平与内源性5-HT-d0水平的比率，以及TA-d2与TA-d0的比率。得出的结论是，5-HT的尿排泄可能反映其在中枢神经系统中的周转，而TA的尿排泄可能反映其外周周转。
