脂肪酸甲酯在弱极性毛细管柱上的气相色谱分离
Gas chromatographic separation of fatty acid methyl esters on weakly polar capillary columns.
作者信息
Yamamoto Kouhei, Kinoshita Akemi, Shibahara Akira
机构信息
Department of Clinical Nutrition, Osaka Prefecture University, 7-30 Habikino 3-chome, Habikino 583-8555, Japan.
出版信息
J Chromatogr A. 2008 Feb 22;1182(1):132-5. doi: 10.1016/j.chroma.2007.12.071. Epub 2008 Jan 6.
It was found that weakly polar columns, routinely used in capillary GC for analyzing sterols, food additives, etc., can also be used for separating fatty acid methyl esters (FAMEs). On these columns, FAMEs elute in the order of their unsaturation. The equivalent chain-length value of methyl 22:6 is below 23.00. This means FAMEs within a carbon chain length, having up to six double bonds, elute before the next (one carbon longer) saturated FAME elutes. Peak identification is easy. Weakly polar columns are compatible in both GC and GC/MS systems.
研究发现，毛细管气相色谱中常规用于分析甾醇、食品添加剂等的弱极性柱，也可用于分离脂肪酸甲酯（FAMEs）。在这些柱上，FAMEs按不饱和度顺序洗脱。22:6甲酯的等效链长值低于23.00。这意味着在一个碳链长度内、具有多达六个双键的FAMEs，会在下一个（长一个碳）饱和FAME洗脱之前先洗脱。峰的鉴定很容易。弱极性柱在气相色谱和气相色谱/质谱系统中均适用。
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