Saito Keita, Okamura Kota, Kataoka Hiroyuki
School of Pharmacy, Shujitsu University, Nishigawara, Okayama 703-8516, Japan.
J Chromatogr A. 2008 Apr 4;1186(1-2):434-7. doi: 10.1016/j.chroma.2007.12.078. Epub 2008 Jan 6.
A simple and sensitive method for the determination of musty odorants, 2-methylisoborneol (MIB) and geosmin (GSM), in environmental water was developed by headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry. MIB and GSM were separated within 10 min using a DB-1 capillary column and detected in the selective ion monitoring mode. HS-SPME using a polydimethylsiloxane/divinylbenzene fiber provided effective sample enrichment, and was carried out by fiber exposition at 70 degrees C for 30 min. Using this method, the calibration curves of MIB and GSM were linear in the range of 0-500 pg/mL, with a correlation coefficient above 0.9977 (n=24). The detection limits (S/N=3) of MIB and GSM were 0.9 and 0.6 pg/mL, respectively. This method was successfully applied to the analysis of environmental water samples without interference peaks.
建立了一种顶空固相微萃取（HS-SPME）结合气相色谱-质谱联用测定环境水中霉味物质2-甲基异冰片（MIB）和土臭素（GSM）的简单灵敏方法。使用DB-1毛细管柱在10分钟内分离MIB和GSM，并采用选择离子监测模式进行检测。使用聚二甲基硅氧烷/二乙烯基苯纤维的HS-SPME实现了有效的样品富集，通过在70℃下将纤维暴露30分钟来进行。采用该方法，MIB和GSM的校准曲线在0-500 pg/mL范围内呈线性，相关系数高于0.9977（n=24）。MIB和GSM的检测限（S/N=3）分别为0.9和0.6 pg/mL。该方法成功应用于环境水样分析，无干扰峰。
