Vareková Radka Svobodová, Bradác Ivan, Plchút Martin, Skrdla Michal, Wacenovsky Michael, Mahr Helmuth, Mayer Georg, Tanner Herbert, Brugger Hermann, Withalm Josef, Lederer Peter, Huber Heinrich, Gierlinger Gerhard, Graf Ronald, Tafer Hakim, Hofacker Ivo, Schuster Peter, Polcík Martin
ANF DATA (Siemens), Herspická 5, 639 00 Brno, Czech Republic.
Comput Methods Programs Biomed. 2008 Apr;90(1):89-94. doi: 10.1016/j.cmpb.2007.12.001. Epub 2008 Jan 22.
RNA interference (RNAi) has become an important tool to study and utilize gene silencing by introducing short interfering RNA (siRNA). In order to predict the most efficient siRNAs, a new software tool, RNA Workbench (RNAWB), has been designed and is freely available (after registration) on http://www.rnaworkbench.com. In addition to the standard selection rules, RNAWB includes the possibility of statistical analyses of the applied selection rules (criteria). The role of RNA secondary structures in the RNA interference process as well as the application of sequence rules are discussed to show the applicability of the software.
RNA干扰（RNAi）已成为通过引入小干扰RNA（siRNA）来研究和利用基因沉默的重要工具。为了预测最有效的siRNA，已设计了一种新的软件工具RNA工作台（RNAWB），可在http://www.rnaworkbench.com上免费获取（注册后）。除了标准的选择规则外，RNAWB还包括对应用的选择规则（标准）进行统计分析的可能性。讨论了RNA二级结构在RNA干扰过程中的作用以及序列规则的应用，以展示该软件的适用性。
