Caccamo Daniela, Di Mauro Debora, Condello Salvatore, Currò Monica, Cutroneo Giuseppa, Anastasi Giuseppe Pio, Ientile Riccardo, Trimarchi Fabio
Department of Biochemical, Physiological and Nutritional Sciences, University of Messina, Via C. Valeria, Policlinico Universitario, 98125, Messina, Italy.
Amino Acids. 2009 Jan;36(1):49-56. doi: 10.1007/s00726-008-0025-x. Epub 2008 Jan 22.
Several studies demonstrated that transglutaminases play a key role in extracellular matrix stabilization needed for cell differentiation. We evaluated transglutaminase expression and activity in the pre-secretory stage of differentiation of the continuously erupting rat incisor. We observed that transglutaminase-mediated incorporation of monodansylcadaverine into protein substrates was specifically located in the apical loop, and along the basement membrane joining mesenchyme and inner dental epithelium in the odontogenic organ. Enzyme activity was associated with mRNAs for transglutaminase 1 and 2. Notably, labelling cells for these isoenzymes were observed in both mesenchymal and epithelial compartments, but not in the basement membrane, in the ameloblast facing pulp anterior region, where ameloblast and odontoblast differentiation begins. These findings demonstrate that transglutaminase 1 and transglutaminase 2 are expressed at a major extent in the pre-secretory stage of regenerating rat incisor, where they probably play complementary roles in cell signalling between mesenchyme and epithelium and extracellular matrix.
多项研究表明，转谷氨酰胺酶在细胞分化所需的细胞外基质稳定中起关键作用。我们评估了持续萌出的大鼠切牙分化分泌前期转谷氨酰胺酶的表达和活性。我们观察到，转谷氨酰胺酶介导的单丹磺酰尸胺掺入蛋白质底物的过程特异性地位于顶端环，以及牙源性器官中连接间充质和内牙上皮的基底膜沿线。酶活性与转谷氨酰胺酶1和2的mRNA相关。值得注意的是，在成釉细胞面向牙髓的前部区域，即成釉细胞和成牙本质细胞分化开始的地方，在间充质和上皮区室中均观察到了这些同工酶的标记细胞，但在基底膜中未观察到。这些发现表明，转谷氨酰胺酶1和转谷氨酰胺酶2在再生大鼠切牙的分泌前期大量表达，它们可能在间充质和上皮之间的细胞信号传导以及细胞外基质中发挥互补作用。
