Liebano Richard Eloin, Abla Luiz Eduardo Felipe, Ferreira Lydia Masako
Plastic Surgery Division, Surgery Department, Federal University of São Paulo, São Paulo School of Medicine, São Paulo, Brazil.
Wound Repair Regen. 2008 Jan-Feb;16(1):65-9. doi: 10.1111/j.1524-475X.2007.00332.x.
The purpose of this study was to determine the effect of low-frequency (2 Hz) transcutaneous electrical nerve stimulation (TENS) on the viability of ischemic skin flaps in the rat. Seventy-five EPM1-Wistar rats were used. The random skin flap measuring 10 x 4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, animals in all groups were kept anesthetized for 1 hour, with electrodes placed at the base of the flap, and received the assigned treatment. The animals were randomized into five groups (G1-G5) and each group was subjected to the following procedures, which were repeated on the 2 subsequent days: G1-sham stimulation (control); G2-transcutaneous electrical nerve stimulation, TENS (f=2 Hz, I=5 mA), G3-TENS (f=2 Hz, I=10 mA), G4-TENS (f=2 Hz, I=15 mA), G5-TENS (f=2 Hz, I=20 mA). Seven days after treatment, the percentage of flap necrosis was determined. For each group, the mean+/-SEM percentage of flap necrosis was as follows: G1 group (control), 43.88+/-2.02%; G2 group, 39.20+/-3.17%; G3 group, 38.57+/-4.08%; G4 group, 32.14+/-2.89%; and G5 group, 44.13+/-2.98%. The G4 group had the smallest necrotic area compared with the control group. The low-frequency TENS treatment was effective in improving the viability of ischemic skin flap.
本研究的目的是确定低频（2赫兹）经皮电刺激神经疗法（TENS）对大鼠缺血性皮瓣存活能力的影响。使用了75只EPM1-Wistar大鼠。制作了大小为10×4厘米的随意皮瓣，并在皮瓣与其下方组织之间放置了塑料屏障。手术操作后，所有组的动物均保持麻醉状态1小时，电极置于皮瓣基部，并接受指定的治疗。将动物随机分为五组（G1-G5），每组进行以下操作，并在随后的两天重复：G1-假刺激（对照组）；G2-经皮电刺激神经疗法，TENS（频率f = 2赫兹，电流I = 5毫安），G3-TENS（f = 2赫兹，I = 10毫安），G4-TENS（f = 2赫兹，I = 15毫安），G5-TENS（f = 2赫兹，I = 20毫安）。治疗7天后，测定皮瓣坏死的百分比。每组皮瓣坏死的平均百分比±标准误如下：G1组（对照组），43.88±2.02%；G2组，39.20±3.17%；G3组，38.57±4.08%；G4组，32.14±2.89%；G5组，44.13±2.98%。与对照组相比，G4组的坏死面积最小。低频TENS治疗可有效提高缺血性皮瓣的存活能力。
