Boucrot Emmanuel, Kirchhausen Tomas
Department of Cell Biology and Immune Disease Institute, Harvard Medical School, Boston, Massachusetts, USA.
PLoS One. 2008 Jan 23;3(1):e1477. doi: 10.1371/journal.pone.0001477.
Using single cell-imaging methods we have found that the volume of adherent cells grown in culture decreases as the cells rounds when it enters mitosis. A minimal volume is reached at metaphase. Rapid volume recovery initiates before abscission as cells make the transition from metaphase to cytokinesis. These volume changes are simultaneous with the rapid surface area decrease and recovery observed in mitotic cells [1].
使用单细胞成像方法，我们发现培养中生长的贴壁细胞在进入有丝分裂时变圆，其体积会减小。在中期达到最小体积。随着细胞从中期向胞质分裂转变，在细胞脱离之前就开始了快速的体积恢复。这些体积变化与有丝分裂细胞中观察到的表面积快速减小和恢复同时发生[1]。
